|Related Categories||Core Bioreagents, Life Science Reagents for Protein Electrophoresis, Markers, Molecular Biology, Molecular Weight Markers,|
|usage||sufficient for 10-20 large gels (16 × 18 cm)|
|sufficient for 20-30 mini-gels (10 × 10 cm)|
|1 vial sufficient for 50-100 PhastGels|
|mol wt||mol wt 6,500-66,000 Da|
SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.
Protein (mol. wt.)
Aprotinin, bovine lung (6,500)
α-Lactalbumin, bovine milk (14,200)
Trypsin inhibitor, soybean (20,000)
Trypsinogen, bovine pancreas (24,000)
Carbonic anhydrase, bovine erythrocytes (29,000)
Ovalbumin, chicken egg (45,000)
Albumin, bovine serum (66,000)
Will yield eight protein bands with Coomassie Blue staining.
Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue.
SigmaMarker is a trademark of Sigma-Aldrich Co. LLC
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mol wt 8,000-220,000 Da
high range, mol wt 36,000-200,000 Da
wide range, mol wt 6,500-200,000 Da
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