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M4159 Sigma

Methylene Blue hydrate

suitable for nucleic acid staining, BioReagent

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Properties

Related Categories Cell Biology, Hematology and Histology, M, Stains & Dyes, A to Z, Stains and Dyes More...
grade   for molecular biology
InChI Key   WQVSELLRAGBDLX-UHFFFAOYSA-M
product line   BioReagent
form   powder
suitability   suitable for nucleic acid staining
storage temp.   room temp

Description

Features and Benefits

• Less hazardous than staining with ethidium bromide
• Viewable in visible light
• Fast and easy to use

General description

Methylene blue is a heterocyclic aromatic chemical, appearing as a dark green solid powder, changing to blue in aqueous solution. It is used as a stain in many different histology procedures, as well as in molecular biology laboratories.

Packaging

25, 100 g in glass bottle

Application

Methylene Blue hydrate is suitable for use as a nucleic acid stain for agarose and polyacrylamide gel electrophoresis (PAGE). It was used to stain 4T1 tumor cells to study metastasis of tumor cells using clonogenic assay. Methylene blue hydrate crystals were encapsulated in apoferritin nanocages for photodynamic therapy against tumor cells.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Methylene Blue - Molecular Biology Reagent

Prepare a staining solution containing 0.2% (w/v) methylene blue in 0.4 M sodium acetate:0.4 M acetic acid (1:1). Use enough staining solution to cover the gel. Remove the staining solution after 1 h...

Peer-Reviewed Papers
15

References

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M4159 Methylene Blue hydrate, suitable for nucleic acid staining, BioReagent

Technical Service:

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