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M4171 Sigma

Melittin

≥97% (HPLC), synthetic

  • CAS Number 20449-79-0

  • Empirical Formula (Hill Notation) C131H229N39O31

  • Molecular Weight 2846.46

  •  MDL number MFCD00076868

  •  PubChem Substance ID 24896910

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Properties

InChI Key   VDXZNPDIRNWWCW-JFTDCZMZSA-N
assay   ≥97% (HPLC)
storage temp.   −20°C
Gene Information   human ... ATP1A1(476), CALM1(801)
mouse ... ATP1A1(11928), CALM1(12313)
rat ... ATP1A1(24211), CALM1(24242)

Description

Amino Acid Sequence

Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH2

Biochem/physiol Actions

Binds calmodulin in a Ca2+-dependent manner; inhibits Na+-K+-ATPase.

Melittin acts as an anti-coagulating protein by increasing the time of blood clotting in vitro. Melittin inhibits the activity of S100 calcium-binding protein B (S100B) and plays a vital role in Epilepsy treatment. Melittin retard cathepsin S-induced invasion, proliferation and angiogenesis via inhibition of the vascular endothelial growth factor A (VEGF-A) /VEGF receptor 2 (VEGFR-2)/ mitogen-activated protein kinase 1 (MEK1)/ extracellular signal-regulated kinase 1/2 (ERK1/2) pathway in human MHCC97-H cells (liver cancer cells). Melittin has therapeutic application in various inflammatory diseases such as skin inflammation, neuroinflammation, atherosclerosis, arthritis and liver inflammation. Accumulated melittin degrades phospholipid packing in the cell membrane and causes cell lysis and cell death.

Other Notes

The principle hemolytic component of honeybee venom.

Lyophilized from 0.1% TFA in H2O

Packaging

1 mg in poly drum

250 μg in glass bottle

Application

Melittin has been used in standard bacterium-killing assay to determine bactericidal activity and study the role of melittin in allergic reactions.

General description

Melittin is hydrophobic in nature except for a region with Lys-Arg-Lys-Arg sequence near C-terminal end. This structural characteristic makes melittin a highly surface-active and a powerful, direct hemolytic agent.The encoed protein contains 26 amino acids. Monomeric form of melittin has a molecular weight of 2,840Da and tetrameric form has molecular weight of approximately 12,500Da.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
UN 3462 6.1 / PGI
WGK Germany 
3
RTECS 
OS3965000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

HPLC Analysis of Peptide Separation on BIOshell™ A400 Protein C4 at Elevated Temperature

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptide Separation on BIOshell™ A400 Protein C4 at Elevated Temperature
Keywords: High performance liquid chromatography

Peer-Reviewed Papers
15

References

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