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  • M4276 - Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse

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M4276 Sigma

Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse

clone MY-32, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   feline, rat, bovine, chicken, human, mouse, rabbit, guinea pig
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using skeletal muscle tissue
  indirect immunofluorescence: 1:400
  western blot: 1:1,000 using rabbit leg muscle extract
clone   MY-32, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MYH1(4619), MYH2(4620)
mouse ... Myh1(17879), Myh2(17882)
rat ... Myh1(287408), Myh2(691644)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

rabbit muscle myosin.

General description

Localizes an epitope on the myosin heavy chain. Stains the fast (type II) and neonatal isomyosin molecules found in skeletal muscle, but does not stain cardiac muscle, smooth muscle or non-muscle myosin in cultured cells. Does react with human rhabdomyosarcomas.

Myosin is a 480,000 dalton protein known to interact with actin in muscle and in non-muscle cells. It contains two identical heavy chains (200,000 daltons each) and four light chains (15,000-26,000 daltons). Myosin molecules consist of two major regions: tails (rods) and heads; they aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Multiple forms of myosin heavy chains exist for each muscle type-skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle. These are designated at type I (slowtwitch) and type II (fast-twitch). Type II fibers can be further subdivided in types IIA, IIB, and IIC.

Specificity

Monoclonal Anti-Skeletal Myosin is specific for the myosin heavy chain. It does not stain human or animal cardiac or smooth muscle myosin or cells grown by tissue culture (nonmuscle myosin). It has been demonstrated on human skeletal muscle that the antibody stains the fast twitch (type II) isomyosin molecules. Monoclonal Anti-Skeletal Myosin antibody does react with human rhabdomyosarcomas.

Application

Monoclonal Anti-Skeletal Myosin may be used for staining of human, rabbit, rat, mouse, bovine, chicken and guinea pig skeletal myosin. Monoclonal Anti-Skeletal Myosin antibody to fast-twitch skeletal myosin may be used for detecting cross striated muscle differentiation in tumors. The antibody localizes an epitope on the myosin chain that is stable to the routine formalin-fixation and paraffin-embedding process. Monoclonal Anti-Myosin (Skeletal, Fast) antibody may be used with immunoperoxidase or immunofluorescent procedures on formalin-fixed or frozen sections of skeletal muscle as well as with immunoblotting or dot immunobinding on muscle extracts or purified myosin preparations.
The level of mysosin (fast) in serum samples from sportsmen with past injury was determined by western blot using monoclonal mouse anti-myosin (skeletal/fast) as the primary antibody at a dilution of 1:90000.
A minimum working dilution of 1:400 is determined by indirect immunofluorescent labeling of formalin-fixed, paraffin-embedded sections of human or animal skeletal muscle tissue preparation.
A minimum working dilution of 1:1,000 is determined by immunoblotting using rabbit leg muscle extract.

The level of mysosin (fast) in serum samples from sportsmen with past injury was determined by western blot using monoclonal mouse anti-myosin (skeletal/fast) as the primary antibody at a dilution of 1:90000.

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