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M4280 Sigma

Anti-Mouse IgG (Fc specific) antibody produced in goat

2.0 mg/mL, affinity isolated antibody

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Properties

Related Categories Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates, Primary Antibodies,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
concentration   2.0 mg/mL
application(s)   indirect ELISA: 1:20,000
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Immunogen

Fc fragment of IgG purified from mouse.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-mouse IgG (Fc specific) antibody can be used in indirect ELISA and immunoelectrophoresis.

General description

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. The antigen binding activity is mediated by the variable Fab region of IgG antibodies, whereas the effector activities are stimulated by the Fc domain. Anti-mouse IgG (Fc specific) antibody can be used to detect the optimal capture agents. Goat anti-mouse IgG (Fc specific) antibody reacts specifically with IgG of mouse but does not react with other mouse immunoglobulins, bovine IgG and IgM, horse IgG or human IgG, IgA, IgM, κ and λ light chain.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

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