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M4308 Sigma

Monoclonal Anti-MDM2 antibody produced in mouse

clone SMP14, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to E1,E2,E3 Ligase,
species reactivity   human, rat, mouse (weak)
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1:1,000 using a whole extract of transfected 293T (human embryonal kidney) cells expressing human MDM2
clone   SMP14, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt ~90 kDa (additional band of approx. 55 kDa)
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MDM2(4193)
mouse ... Mdm2(17246)
rat ... Mdm2(246362)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 154-167 of human MDM2 conjugated to KLH.

Specificity

Detects the MDM2 protein alone or in complex with E2F1, p53, and retinoblastoma proteins. A slight cross-reactivity is observed with cytokeratins 6, 14, and 16.

Price and Availability


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