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  • M4401 - Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse

M4401 Sigma

Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse

clone MY-21, ascites fluid


Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   chicken, bovine, pig, rabbit, human
application(s)   indirect immunofluorescence: 1:200 using human or chicken fibroblasts
  western blot: suitable using denatured and reduced myosin of muscle
clone   MY-21, monoclonal
antibody form   ascites fluid
isotype   IgM
mol wt   antigen mol wt 20 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   chicken ... MYLK2(396356)
human ... MYLK2(85366)
biological source   mouse
conjugate   unconjugated



chicken lens membrane.

General description

Myosin is a cytoskeletal protein responsible for muscle contraction, muscle filament sliding and regulation of ATPase activity. Monoclonal anti-myosin (Light Chains 20 kDa) antibody diluted 1:200 can be used to detect the extent of phosphorylation of MLC20. It is also useful in staining (1:10,000) 2D slab gels. Anti-myosin(Light Chains 20 kDa) antibody reacts specifically with 20 kDa light chain of chicken gizzard, chicken intestine and pig stomach myosin which is SDS, heat denatured and reduced but not with intact myosin molecule in its native state.


Monoclonal anti-myosin (Light Chains 20 kDa) antibody can be used in immunoblotting and western blotting.

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