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M4439 Sigma

Monoclonal Anti-c-Myc antibody produced in mouse

clone 9E10, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-c-Myc

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Properties

Related Categories c-myc, Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies for Cell Biology,
species reactivity   human
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunoprecipitation: suitable
  microarray: suitable
  western blot: 1:5000 using c-myc tagged fusion protein
clone   9E10, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
mol wt   mol wt 62 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MYC(4609)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.

General description

c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. It belongs to the family of transcription factors which are characterized by basic region helix-loop-helix/leucine zipper. Along with N-Myc and L-Myc, it forms the MYC proto-oncogene family. It forms a heterodimer with MAX, and this heterodimer binds to the E-boxes/CACGTG sequence motif on DNA. This gene is localized to human chromosome 8q24.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Specificity: N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

Preparation Note

Working dilution: 1:5000

Application

Monoclonal Anti-c-Myc antibody produced in mouse is suitable for immunoprecipitation, immunoblotting, ELISA, immunofluorescence, microarray and electron microscopy. It has also been used in ChIP (Chromatin Immunoprecipitation).

Biochem/physiol Actions

v-myc avian myelocytomatosis viral oncogene homolog belongs to MYC family. When overexpressed these proteins can cause DNA double-stand breaks (DSBs) and genome instability. This transcription factor MYC helps in regulating long noncoding RNAs (lncRNAs) which has been implicated in cancer cell proliferation and tumorigenesis. Its overexpression is also associated with various cancers, and in particular B cell lymphomas. Increased expression of c-MYC has a negative effect on the ability of B cell lymphomas to functionally present Ags/peptides to CD4+ T cells. This protein exerts suppressive effects at several critical checkpoints in Ag presentation. It is associated with Burkitt lymphoma and a group of diffuse large B-cell lymphoma (DLBCL).

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RIDADR  NONH for all modes of transport
WGK Germany  3
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