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M4684 Sigma

Membrane Transport: A Practical Approach

  •  ISBN-10 0-19-963704-0

  •  ISBN-13 978-0-19-963704-1

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Related Categories Biochemistry Books, Books and Software, Labware More...
publication info   S.A. Baldwin, ed., Oxford University Press, 2000, 352 pp., soft cover
mfr. no.   Oxford University Press

Description

General description

This book will be of interest to those working on the mechanism of solute transport across biological membranes. Its scope is broad, ranging from the techniques required to study transport itself, through to the expression, purification and reconstitution of transporters, and techniques for investigation of their structure. It includes computer prediction of transporter topology and structure. Most of the protocols can be implemented in any well-found laboratory.

Table of Contents

Assay of membrane transport in cells and membrane vesicles
Reconstitution of membrane proteins: the Ca2+-ATPase of
sarcoplasmic reticulum
The Xenopus oocyte expression system for the cDNA cloning and characterization of plasma membrane transport proteins
Expression of foreign transport proteins in yeast
Baculovirus-mediated overexpression of transport proteins
The amplified expression, identification purification, assay and
properties of hexahistidine-tagged bacterial membrane transport proteins
Spectroscopic and kinetic approaches for probing the mechanisms of solute transporters
Detection and analysis of glucose transporters using photolabelling techniques
Computer prediction of transporter topology and structure
Two-dimensional crystallization of membrane proteins

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