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M4782 Sigma

Mutanolysin from Streptomyces globisporus ATCC 21553

aseptically filled, lyophilized powder, ≥4000 units/mg protein (biuret)

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Properties

Related Categories Bacterial, Biochemicals and Reagents, Cell Biology, Cell Lysis Reagents, Cell Lysis Reagents Enzymes,
sterility   aseptically filled
form   lyophilized powder
mol wt   mol wt 23 kDa
shipped in   wet ice
storage temp.   −20°C

Description

General description

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Preparation Note

Prepared from M 9901

Unit Definition

One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Physical form

Lyophilized powder containing Ficoll and sodium succinate buffer salts

Application

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Biochem/physiol Actions

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products. The Continuous Spectrophotometric Rate Determination (A600, Light path = 1 cm) is based on the following reaction:

Related Content

Enzyme Explorer Key Resources: Lysing Enzymes

Cell Lysis Enzymes – How to tear down the Wall Enzymes provide a non-mechanical method for cell lysis and protoplast preparation. It may seem like a simple process to throw in your enzyme, stick your...
Keywords: Amplification, Biochemistry, Cell culture, Cell disruption, Culture media, Enzymology, Metabolites, Molecular biology, PAGE, Purification

Peer-Reviewed Papers
15

References

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substrate

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M3440 Mutanolysin Assay Substrate, (Lyophilized suspension of washed Enterococcus faecalis STF-3 (ATCC 12984) cell walls.)

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