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M4892 Sigma

McCoy’s 5A Medium

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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Properties

Related Categories Cell Culture, Classic Media and Salts, McCoy’s 5A Modified Medium, Powder Media
Quality Level   GMP
form   powder
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Application

Used for the primary culture of cells from a wide range of tissues. Recommend use for production of virus in primary cell cultures.

Biochem/physiol Actions

Originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also be used in the culture of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Quantity

Formulated to contain 11.9 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Price and Availability


All labs need water

Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Peer-Reviewed Papers
15

References

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