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  • M5177 - Anti-Matrix Metalloproteinase-9, C-Terminal antibody produced in rabbit

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M5177 Sigma

Anti-Matrix Metalloproteinase-9, C-Terminal antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-MMP-9

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Adhesion, Attachment, and Matrix Factors, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   human
application(s)   western blot: 1:1,000 using concentrated cell culture medium from a stimulated human cell line (M2928)
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MMP9(4318)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminal end of human matrix metalloproteinase-9 (gelatinase-B).

General description

Matrix metalloproteinase-9 (MMP-9) is a zinc-dependent proteinase which is also known as 92kDa gelatinase-B. It is expressed by various cells like granulocytes and mononuclear cells. The gene encoding it is localized on human chromosome 20q11.2-q13.1 and has 13 exons.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 50% glycerol and 0.05% sodium azide.

Specificity

By immunoblotting against the reduced protein, the antibody reacts with bands at 92 kDa and 88 kDa (the pro-form and active form). The antibody performs best on reduced proteins, but will react with non-reduced MMP-9. When used on reduced samples from tissue culture media, the antibody also recognizes a band at 65 kDa, which may be removed by enriching the gelatinase on gelatin-agarose.

Biochem/physiol Actions

Matrix metalloproteinase-9 (MMP-9) has been studied as a biomarker of nervous tissue inflammation in multiple sclerosis (MS).

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Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  1
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