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M5284 Sigma

IgG1 Isotype Control from murine myeloma

clone MOPC 21, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Analytical Cytology, Antibodies, Antibody Characterization and Analysis, Biochemicals and Reagents, Isotype Controls,
biological source   mouse
antibody form   purified immunoglobulin
clone   MOPC 21, monoclonal
form   buffered aqueous solution
application(s)   flow cytometry: suitable
conjugate   unconjugated
shipped in   wet ice
storage temp.   2-8°C

Description

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Application

IgG1 isotype control antibodies are suitable for use in immunohistochemistry (7μg/ml) and immunoblotting. The product may also be used for flow cytometry.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . IgG1 isotype control antibody is specific for anti-mouse whole serum and anti-mouse IgG1. The product does not react with antisera to mouse IgA, IgM, IgG2a, IgG2b, and IgG3.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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