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M5293 Sigma

Monoclonal Anti-Mucin Gastric antibody produced in mouse

clone 45M1, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix, Antibodies to Extracellular Matrix Proteins,
species reactivity   mouse, rat, monkey, hedgehog, pig, rabbit, human, feline, chicken
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using sections of human stomach
  western blot: suitable using non-reducing conditions
clone   45M1, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MUC5AC(4586)
mouse ... Muc5ac(17833)
rat ... Muc5ac(65188)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

mucin from human ovarian cyst fluid.

General description

Mucin has a netlike, branched structure and is a secreted protein. It belongs to the category of gel-forming mucins. The gene encoding this protein is upregulated by interleukin-1β (IL-1β) and is localized on chromosome 11p15.5.

Specificity

The antibody recognizes the mucin epitope g located in the peptide core of gastric mucin (>1,000 kDa). This epitope is completely destroyed by thiol reduction (using mercaptoethanol) and partially lost following trypsin proteolysis, but is stable upon periodate oxidation. The antibody stains the surface gastric epithelium of normal human gastrointestinal tract and reacts with fetal, pre-cancerous and cancerous colonic mucosa, but not with normal colon.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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