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M5313 Sigma

Molecular Biology Problem Solver: A Laboratory Guide

  •  ISBN-10 0-471-37972-7

  •  ISBN-13 978-0-471-37972-0

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publication info   A.S. Gerstein, ed., Wiley-Liss, 2001, 528 pp., soft cover
mfr. no.   Wiley

Description

General description

This is a unique resource that will strengthen your ability to troubleshoot experiments by providing a firm basis for understanding how things work and what can go wrong. The materials serve a framework on which to build strong analytical bench skills. Coverage includes working with hazardous materials, calculations and reagent preparation. Clarifying theory and practice, the focus is on the critical elements of techniques, reagents and procedures.

Table of Contents

Planning for Success in the Lab
Getting What You Need from a Supplier
The Preparation of Buffers and Other Solutions: A Chemist′s Perspective
How to Properly Use and Maintain Laboratory Equipment
Working Safely with Biological Samples
Working Safely with Radioactive Materials .
DNA Purification
RNA Purification
Restriction Enzymes
Nucleotides, Oligonucleotides, and Polynucleotides
PCR .
Electrophoresis
Western Blotting
Nucleic Acid Hybridization
Prokaryotic Expression Systems .
Eukaryotic Expression Systems

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