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M5379 Sigma

L-Methionine sulfoximine

Synonym: L-S-(3-Amino-3-carboxypropyl)-S-methylsulfoximine

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Excitatory Amino Acids, Neuroscience, Neurotransmission,
Quality Level   PREMIUM
InChI Key   SXTAYKAGBXMACB-DPVSGNNYSA-N
mp   >210 °C (dec.)(lit.)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1, 5 g in poly bottle

250, 500 mg in poly bottle

Application

As a potent inhibitor of glutamine sythetase activity, this reagent has widely been used as a selection agent for plasmid integration in Chinese hamster ovary (CHO) and other mammalian cell lines. The growing demand for high yield cell banks for production of recombinant proteins for therapeutics has resulted in two major systems for selection of stable and active clones, Methotrexate selection of dihydrofolate reductase (DHFR) overexpressing cells and MSX selection of glutamine synthetase overexpressing cells. Cells are grown in the absence of glutamine in the media and inhibition of the endogenous activity of glutamine synthease results in cell death for cells lacking overexpression. The MSX-glutamine synthase selection mechanism provides benefits over that of the Methotrexate-DHFR system in that it typically requires a single amplification step and results in significant reduction of time to produce high stability, highly amplified clones.

Biochem/physiol Actions

Methionine sulfoximine (MSX) increases ornithine decarboxylase activity, decreases the survival rate in a model of transient cerebral ischemia, and inhibits glutamine synthetase activity.

Features and Benefits

This compound is also offered as part of Sigma′s Library of Pharmacologically Active Compounds (LOPAC®1280), a biologically annotated collection of high-quality, ready-to-screen compounds. Click here to learn more.

Legal Information

LOPAC is a registered trademark of Sigma-Aldrich Co. LLC

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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

MSMLS Online Plate Map

Metabolite descriptors included with the software download upon purchase of the product include: Name, Parent CID, molecular formula, molecular weight, CAS, ChEBI, HMDB ID, PubChem Compound and Subst...
Keywords: Mass spectrometry, Metabolites

Peer-Reviewed Papers
15

References

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