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M5409 Sigma

Mouse IgG2a Isotype Control from murine myeloma

clone UPC-10, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Analytical Cytology, Antibodies, Antibody Characterization and Analysis, Biochemicals and Reagents, Isotype Controls,
biological source   mouse
antibody form   purified immunoglobulin
clone   UPC-10, monoclonal
form   buffered aqueous solution
application(s)   flow cytometry: suitable
shipped in   wet ice
storage temp.   2-8°C

Description

Specificity

When evaluated in flow cytometry, the product did not stain human peripheral blood lymphocytes (PBLs). Anti-Mouse IgG (whole molecule), F(ab’)2 fragment FITC, Catalog Number F2883, along with 1 mg of the product was incubated with human PBLs and then evaluated by flow cytometry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Mouse IgG2a Isotype Control antibodies can be used for affinity chromatography and chromatin immunoprecipitation . The product can also be used for flow cytometry.

Mouse IgG2a Isotype Control clone UPC-10 is recommended as a negative control in flow cytometry. Working Concentration: equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Mouse IgG2a antibodies react with anti-mouse whole serum and anti-mouse IgG2a. The antibody product can be used as controls in immunoassays.

Isotype controls are non-reactive immunoglobulins of the same isotype as the primary antibody being used in an application. It is recommended that a non-reactive immunoglobulin of the same isotype and concentration be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

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