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M5437 Sigma

Anti-MSK-1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, MS-MUD,
species reactivity   rat
application(s)   immunocytochemistry: 1:1,000 using methanol/acetone-fixed rat fibroblast Rat-1 cell line
  microarray: suitable
  western blot: 1:2,000 using using whole cell lysate of rat fibroblast Rat-1 cell line
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 90 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... RPS6KA5(9252)
rat ... Rps6ka5(445349)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminus of human MSK1 (amino acids 783-800). This sequence is not found in the human and mouse MSK2 isoforms.

General description

The mitogen- and stress-activated kinases, MSK-1 and MSK-2 are activated by ERK/MAPK and p38 MAPK in response to growth and cell stress stimuli. MSK-1 and MSK-2 have 75% homology and interact with ERK1/2 and p38 through MAPK-binding domains near the C-terminus. There is a predominant expression of MSK proteins in heart, brain, skeletal muscles, lung, liver, kidney, pancreas and placenta. Highly conserved phosphorylation sites of MSK-1, Ser260 and Thr581, are followed by proline residues indicating that MSK-1 is activated by more than one MAPK. Endogenous MSK-1 is activated in cells by growth factors, phorbol ester stimulation, exposure to UV irradiation, and oxidative or chemical stress and is localized to nucleus of unstimulated or stimulated cells. MSK-1 is directly involved in the regulation of gene expression since it actively phosphorylates, stabilizes and activates many transcription factors. The major transcription factors that are regulated by MSK-1 include CREB, NF-κB and STAT3. MSKs also evidently phosphorylate Histone H3 at Ser10 and Ser28 and HMG at Ser6. Thus a primary role of MSK proteins is also promotion of gene relaxation and activation by regulating the binding of transcription repressors. Anti-MSK-1 specifically recognizes rat MSK-1 (90 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-MSK-1 may be used at a minimum working dilution of 1:2000 (immunoblotting in whole cell extracts) or 1:1000 (immunocytochemistry) in fibroblast Rat1 cell line. The antobody has also been used for detection by immunoblotting in mouse fibroblast 10T1/2 cells.

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