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M5505 Sigma

Silver Stain SDS-PAGE Molecular Weight Standard Mixture

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Properties

Related Categories Molecular Biology, Molecular Weight Markers, Protein Electrophoresis, Proteomics
form   aqueous glycerol solution
usage   sufficient for 300 standard gels (16 × 18 cm)
  1 vial sufficient for 600 mini-gel applications
shipped in   dry ice
storage temp.   −20°C

Description

Application

Designed for molecular weight determinations on silver stained SDS-PAGE (Laemmli) gels. The proteins supplied in these markers cover the range of molecular weights common to most proteins or their subunits. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity with low background after electrophoresis and staining using either ProteoSilver (PROTSIL1) or ProteoSilver Plus (PROTSIL2).

Other Notes

Will yield 5 protein bands with silver staining:
• β-Galactosidase, E. coli, 116,000 Da
• Phosphorylase b, Rabbit Muscle, 97,400 Da
• Serum Albumin, Bovine, 66,000 Da
• Fumarase, Porcine Heart, 48,500 Da
• Carbonic Anhydrase, Bovine Erythrocytes, 29,000 Da

Packaging

Vial contains 200 μl of protein markers. Supplied with one vial of 2× Laemmli sample buffer (S3401).

Reconstitution

Dilute the concentrate between 1:15 and 1:30 depending on the amount of protein loaded in the other lanes. Recommended loading volumes are 10 μl per well for mini gels (10 × 10 cm) or 20 μl per well for standard format gels (16 × 18 cm).

Physical form

Solution in 300 mM NaCl, 100 mM DTT, 3 mM NaN3, and 50% glycerol

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles
Peer-Reviewed Papers
15

References

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