• USA Home
  • M5519 - Murashige and Skoog Basal Medium


M5519 Sigma

Murashige and Skoog Basal Medium

powder, plant cell culture tested

Synonym: MS Basal Medium



Related Categories Culture Media, Salts and Vitamin Mixes, Molecular Biology, Plant Biotechnology, Plant Tissue Culture
form   powder
suitability   plant cell culture tested
Featured Industry   Agriculture
storage temp.   2-8°C


Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Formula variant

With the macro- and micronutrients, and vitamins as described by Murashige and Skoog (1962).

Media Formulation

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.


Formulated to contain 4.4 grams of powder per liter of medium.


Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Price and Availability

Timestrip - Time & Temperature Indicators

Discover how Timestrip® indicators will help you monitor expiry dates or excessive temperatures.

Additional Products to Explore

3-Indoleacetic acid

plant cell culture tested, crystalline


plant cell culture tested

for molecular biology

BioReagent, plant cell culture tested, powder


BioXtra, ≥99.5% (GC)

Customers Also Viewed

powder, plant cell culture tested, with Gamborg′s vitamins

plant cell culture tested, with sucrose and agar

10 ×, liquid, plant cell culture tested

powder, plant cell culture tested

powder, plant cell culture tested

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Hazard Codes (Europe) 
Risk Statements (Europe) 
Safety Statements (Europe) 
UN 1477 5.1 / PGII
WGK Germany 

Protocols & Articles

Related Content

Agriculture | Crop Protection Workflow

Pesticides are an essential component in modern agriculture and enable growers to fully realize the yield potential of their seeds. From compound discovery to active ingredient development, our chemi...
Keywords: Agriculture, Pesticides

Plant Tissue Culture Products | Agriculture

Our combination of quality products, services and global business capabilities help you to maintain reagent consistency while ensuring suitability. Sigma-Aldrich allows you to standardize reagents ac...
Keywords: Antibiotics, Cell culture, Culture media, Gas chromatography, High performance liquid chromatography, Mass spectrometry, Plant biotechnology, Vitamins, transformation

Peer-Reviewed Papers


Set your institution to view full text papers.

In vitro cultures and regeneration of Bienertia sinuspersici (Chenopodiaceae) under increasing concentrations of sodium chloride and carbon dioxide. Rosnow J, Offermann S, Park J, et al. Plant Cell Rep., (2011)

Vitrification-based cryopreservation of Grammatophyllum speciosum protocorm. Sopalun K, Kanchit K, Ishikawa K. Cryo. Letters 31, 347-357, (2010)


A revised medium for rapid growth and bioassays with tobacco tissue cultures. Murashige, T., and Skoog, F. Physiol. Plant. 15, 473-497, (1962)


Arabidopsis ribosomal proteins RPL23aA and RPL23aB are differentially targeted to the nucleolus and are disparately required for normal development. Rory F. Degenhardt et al Plant Physiol. 147, 128-42, (2008)


Influence of growth regulators and elicitors on cell growth and α-tocopherol and pigment productions in cell cultures of Carthamus tinctorius L. Chavan SP, Lokhande VH, et. al. Appl. Microbiol. Biotechnol. 89, 1701-1707, (2011)


In vitro adventitious shoot regeneration via indirect organogenesis from petiole explants of Cassia angustifolia Vahl.-a potential medicinal plant. Siddique I, Anis M, Aref IM. Appl. Biochem. Biotechnol. 162, 2067-2074, (2010)


Ectopic expression of class 1 KNOX genes induce adventitious shoot regeneration and alter growth and development of tobacco (Nicotiana tabacum L) and European plum (Prunus domestica L). Srinivasan C, Liu Z, Scorza R. Plant Cell Rep. 30, 655-664, (2011)


Effect of medium composition and light on root and rhinacanthin formation in Rhinacanthus nasutus cultures. Panichayupakaranant P, Meerungrueang W. Pharm. Biol. 48, 1192-1197, (2010)


Assessment of factors affecting micropropagation and ex vitro acclimatization of Nyctanthes arbor-tristis L. Jahan AA, Anis M, Aref IM. Acta Biol. Hung. 62, 45-56, (2011)


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?