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M5519 Sigma

Murashige and Skoog Basal Medium

powder, plant cell culture tested

Synonym: MS Basal Medium

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Properties

Related Categories Culture Media, Salts and Vitamin Mixes, Molecular Biology, Plant Biotechnology, Plant Tissue Culture
form   powder
suitability   plant cell culture tested
Featured Industry   Agriculture
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Formula variant

With the macro- and micronutrients, and vitamins as described by Murashige and Skoog (1962).

Media Formulation

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Application

Murashige and Skoog medium has been used in plant tissue culture medium for plant transformation study.

General description

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
UN 1477 5.1 / PGII
WGK Germany 
2
Protocols & Articles

Protocols

Murashige Skoog - Plant Tissue Culture Protocol

M5524 – Murashige and Skoog basal salt mixture (MS) M0654 – Murashige and Skoog basal salt macronutrient solution M0529 – Murashige and Skoog basal salt micronutrient solution M2909 – Murashige and S...
Keywords: Mass spectrometry, Vitamins

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