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M5519 Sigma

Murashige and Skoog Basal Medium

powder, plant cell culture tested

Synonym: MS Basal Medium

Properties

Related Categories Culture Media, Salts and Vitamin Mixes, Molecular Biology, Plant Biotechnology, Plant Tissue Culture
form   powder
suitability   plant cell culture tested
Market Segment   Agriculture
storage temp.   2-8°C

Description

Frequently Asked Questions

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Formula variant

With the macro- and micronutrients, and vitamins as described by Murashige and Skoog (1962).

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Price and Availability


Plant Tissue Culture

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