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M5546 Sigma

Monoclonal Anti-c-Myc antibody produced in mouse

clone 9E10, ascites fluid

Synonym: Anti-c-Myc

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Embryonic Stem Cells,
species reactivity   human
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 1:100 using c-myc tagged fusion protein
clone   9E10, monoclonal
antibody form   ascites fluid
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MYC(4609)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.

General description

c-Myc is a transcription factor which is over-expressed in a wide range of cancers.

Specificity

Recognizes both N- terminal and C-terminal c-Myc tagged proteins.

Specificity: N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
• Chromatin immunoprecipitation (1 paper)
• Enzyme-linked immunosorbent assay (1 paper)
• Immunoprecipitation (5 papers)
• Western Blotting (5 papers)
• Western blotting following immunoprecipitation(1 paper)

Monoclonal anti-c-Myc antibody produced in mouse has been used for Western blotting and immunohistochemistry.

Biochem/physiol Actions

c-Myc is a regulator of the cell cycle and aids in the growth of tumors in the liver. Translocations in the gene encoding it have been shown to be linked to Burkitt lymphoma. It has been studied that c-Myc targets many protein-coding genes which enhances the oncogenic properties of c-Myc.

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