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M5650 Sigma

Minimum Essential Medium Eagle

With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym: MEM



Related Categories Cell Culture, Classic Media and Salts, Minimum Essential Medium Eagle (MEM) More...
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
storage temp.   2-8°C



Contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.


Supplement with 0.292 g/L L-glutamine.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

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Price and Availability

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Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Related Content

Minimum Essential Medium Eagle - MEM Media

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain sub...
Keywords: Addition reactions, Adhesion, Cell culture, Cloning, Culture media, Eliminations, Metabolism, Vitamins

Peer-Reviewed Papers


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Temporal and embryonic lineage-dependent regulation of human vascular SMC development by NOTCH3. Granata A, Bernard WG, Zhao N, et al. Stem Cells Dev. 24(7), 846-56, (2015)


DNA damage signaling in response to double-strand breaks during mitosis. Giunta S J. Cell Biol. 190(2), 197-207, (2010)


Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells. Wong Yau Hsiung et al Evid. Based. Complement. Alternat. Med. 2011, 293060, (2011)


A critical role for Neurofascin in regulating action potential initiation through maintenance of the axon initial segment. Zonta B Neuron 69(5), 945-56, (2011)


MiR-34a targeting of Notch ligand delta-like 1 impairs CD15+/CD133+ tumor-propagating cells and supports neural differentiation in medulloblastoma. de Antonellis P PLoS ONE 6(9), e24584, (2011)


Molecular and biochemical characterization of a cathepsin B-like protease family unique to Trypanosoma congolense. Mendoza-Palomares C Eukaryotic Cell 7(4), 684-97, (2008)


Degenerate wave and capacitive coupling increase human MSC invasion and proliferation while reducing cytotoxicity in an in vitro wound healing model. Michelle Griffin et al PLoS ONE 6, e23404, (2011)


Identification of DNA regions and a set of transcriptional regulatory factors involved in transcriptional regulation of several human liver-enriched transcription factor genes. Miura H Nucleic Acids Res. 37(3), 778-92, (2009)


Identification of genes potentially involved in supporting hematopoietic stem cell activity of stromal cell line MC3T3-G2/PA6. Natsumi Shimizu et al Int. J. Hematol. 87, 239-45, (2008)


Giardia duodenalis cathepsin B proteases degrade intestinal epithelial interleukin-8 and attenuate interleukin-8-induced neutrophil chemotaxis. Cotton JA, Bhargava A, Ferraz JG, et al. Infect. Immun. 82(7), 2772-87, (2014)


A self-avoidance mechanism in patterning of the urinary collecting duct tree. Davies JA, Hohenstein P, Chang CH, et al. BMC Dev. Biol. 14(1), 35, (2014)


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