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  • M5670 - Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit

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M5670 Sigma

Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit

whole antiserum

Synonym: Anti-MAP Kinase (ERK-1, ERK-2), Anti-Mitogen Activated Protein Kinase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
species reactivity   human, rat, mouse
application(s)   microarray: suitable
  western blot: 1:40,000-1:20,000 using rat brain extract and mouse NIH 3T3 fibroblast extract, respectively
clone   polyclonal
antibody form   whole antiserum
mol wt   antigen ERK-2 mol wt 42 kDa
  antigen, ERK-1 mol wt 44 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK1(5594), MAPK3(5595)
mouse ... Mapk1(26413), Mapk3(26417)
rat ... Mapk1(116590), Mapk3(50689)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 317-339 derived from subdomain XI of human MAP kinase (ERK1).

General description

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.
Anti-MAPK Kinase (ERK-1, ERK-2) specifically reacts with non-phosphorylated form of ERK-1 and ERK-2 (44 and 42 kDa, respectively).

Specificity

Staining of the 42 kDa and 44 kDa bands is specifically inhibited with MAP kinase peptide (317-339), but not with MAP kinase kinase (MEK) peptide (34-48) corresponding to N-terminal sequence of MEK.

Application

Anti-MAPK (ERK-1, ERK-2) antibody may be used at a minimum dilution of 1:40000 for immunoblotting in rat brain extract and at 1:20000 in mouse NIH 3T3 fibroblasts. It may be used for detection of ERK-1/2 in a variety of human cells lines such as HEK293, HaCaT, and human adenocarcinoma, melanoma and fibrosarcoma cells.

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