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M5755 Sigma

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

Synonym: MESA Buffer

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Properties

Related Categories Molecular Biology, Nucleic Acid Electrophoresis, Nucleic Acid Running Buffers, Plant Biotechnology, Plant Molecular Biology More...
grade   for molecular biology
product line   BioReagent
form   powder
pH range   6.8 - 7.2 (reconstituted per directions)
solubility   H2O: soluble, faintly yellow
foreign activity   DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.   room temp

Description

Components

1x MESA buffer contains:
• 40 mM MOPS
• 10 mM sodium acetate
• 1 mM EDTA (pH 8.3)

Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

General description

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Packaging

1 L in poly bottle

Reconstitution

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Peer-Reviewed Papers
15

References

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