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  • M5795 - Anti-MAP Kinase Kinase (MEK, MAPKK) antibody produced in rabbit

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M5795 Sigma

Anti-MAP Kinase Kinase (MEK, MAPKK) antibody produced in rabbit

whole antiserum

Synonym: Anti-MAPKK, Anti-MEK

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, M1-MAR,
species reactivity   rat, mouse, human
application(s)   microarray: suitable
  western blot: 1:20,000 using rat brain extract and mouse NIH 3T3 fibroblast lysate
clone   polyclonal
antibody form   whole antiserum
mol wt   antigen mol wt 45 kDa (MEK1a)
  antigen mol wt 46 kDa (MEK2)
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAP2K1(5604), MAP2K2(5605)
mouse ... Map2k1(26395), Map2k2(26396)
rat ... Map2k1(170851), Map2k2(58960)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 34-48 of human MAP kinase kinase 1 (MEK-1).

General description

MAP Kinase Kinase (MAPKK, MEK1 and MEK2) belong to a family of enzymes that activates their substrate by dual phosphorylation at threonine and tyrosine residues. MEK1 and MEK2 have 86% homology in their catalytic domain. Activation of MEK1/2 is mediated in mitogen-stimulated cells by Raf-1 kinase. MEK1/2 then activates ERK1/2 leading to the activation of several downstream targets that contribute to cell proliferation, apoptosis and motility. In addition to ERK1/2, there may be non-catalytic effectors of MEK1/2. MEK isoforms appear to be widely expressed in the central nervous system, thymus, spleen, heart, lung, and kidney. Active forms of MEK1/2 are sufficient for the transformation of NIH3T3 cells of the differentiation of PC-12 cells.
Anti-MEK reacts in immunoblotting (SDS-PAGE) with MEK-1a (45 kDa protein) and MEK-2 (46 kDa protein), usually forming one band at 46kDa.

Specificity

Reacts with MEK-1a and MEK-2. Staining of MEK-1a and MEK-2 is specifically inhibited with MEK-1 peptide (34-48), but not with MAP kinase peptide (317-399) corresponding to subdomain XI of ERK1.

Application

Anti-MEK may be used for detection by immunoblotting at a working dilution of 1:20,000 in rat brain extract and mouse NIH3T3 fibroblast whole cell lysate. A working dilution of 1:100-1:1000 may be used for immunoblotting in mouse spermatozoa extracts. The antibody is also suitable for protein microarray.

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