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M5814 Sigma

MKK6/SKK3, active human

≥80% (SDS-PAGE), recombinant, expressed in E. coli, Maltose binding protein tagged, buffered aqueous solution

Synonym: MAP Kinase Kinase 6/SKK 3, MAP Kinase Kinase 6

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Properties

Related Categories Active Kinases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes,
recombinant   expressed in E. coli
assay   ≥80% (SDS-PAGE)
form   buffered aqueous solution
mol wt   mol wt 81 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... MAP2K6(5608)

Description

Application

Active enzyme for study of stress-activated protein kinases.

May be used for activation of p38α followed by phosphorylation of MBP.

Specificity

Active in the phosphorylation of p38/SAPK2a

Unit Definition

1 unit will activate 1 unit of p38/SAPK2 per minute. 1 unit of active p38/SAPK2 will incorporate 1 nanomole of phosphorus into myelin basic protein per minute.

Physical form

Solution in approx. 1 mg/ml in 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.1% 2-mercaptoethanol, 0.15 M NaCl, 0.03% Brij® L23, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine.

Legal Information

Brij is a registered trademark of Croda International PLC

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles
Peer-Reviewed Papers
15

References

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