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M5815 Sigma

Monoclonal Anti-Myogenin antibody produced in mouse

clone F12B, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cardiac Stem Cells, Antibodies for Cell Biology, Antibodies for Gene Regulation,
species reactivity   mouse, rat, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using rhabdomyosarcoma tissue sections
  immunoprecipitation: 2 μg using 1 mg protein lysate
  indirect ELISA: suitable
  indirect immunofluorescence: suitable
  western blot: 1 μg/mL
clone   F12B, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 34 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MYOG(4656)
mouse ... Myog(17928)
rat ... Myog(29148)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

rat recombinant myogenin (amino acids 30-224).

General description

Myogenin is a transcription factor present in the nucleus. It possesses an amino-acid rich region and a helix-loop-helix structure. The gene encoding it is localized on human chromosome 1q31-41 and has three exons.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Biochem/physiol Actions

Myogenin acts as a regulator during myoblast and skeletal muscle differentiation. It is considered as a definite marker for skeletal muscle differentiation.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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