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  • M5948 - Anti-MIB1 (C-terminal) antibody produced in rabbit

M5948 Sigma

Anti-MIB1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-DAPK-interacting protein 1, Anti-DIP-1, Anti-KIAA1323, Anti-Mindbomb homolog 1, Anti-Ubiquitin ligase mind bomb


Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to E1,E2,E3 Ligase, Antibodies to Protein Degradation,
species reactivity   rat, human, mouse
application(s)   western blot: 0.5-1 μg/mL using lysates of A549 or PC12 cells.
clone   polyclonal
concentration   ~1.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~110 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MIB1(57534)
mouse ... Mib1(225164)
rat ... Mib1(307594)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 924-939 of human MIB1, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

General description

Mindbomb homolog 1 (MIB1) is an ubiquitin ligase which possesses two substrate recognition domains, ankyrin repeats and an amino terminal zinc finger domain.

Physical form

Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide.


Anti-MIB1 (N-terminal) antibody produced in rabbit has been used for immunofluorescence.

Biochem/physiol Actions

Mindbomb homolog 1 (MIB1) aids in the ubiquitination and internalization of Notch ligands such as the protein δ. It also acts as a positive regulator in the activation of nuclear factor-κB (NF-κB).

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