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M6034 Sigma

IgG2a, Lambda from murine myeloma

clone HOPC-1, purified immunoglobulin, buffered aqueous solution

Synonym: Mouse IgG2a-λ

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Properties

Related Categories Antibodies, Mouse Myeloma Proteins, Purified Immunoglobulins, Sera and Immunoglobulins, Supplementary Products More...
biological source   mouse
antibody form   purified immunoglobulin
clone   HOPC-1, monoclonal
form   buffered aqueous solution
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Physical form

Solution in 0.02 M Tris buffered saline, pH 8.0, containing 0.02% sodium azide

Application

The purified IgG2a, λ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. IgG2a, λ from murine myeloma was used as a standard in ELISA.

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
IgG2a, λ from murine myeloma specifically recognizes mouse IgG2a. Specificity of the mouse IgG2a, λ is determined by immunodiffusion and IEP techniques. The purified myeloma preparation is non-reactive with antisera to mouse IgA, IgM, IgG1, IgG2a, IgG2b and IgG3.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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