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M6065 Sigma

Monoclonal Anti-Microphthalmia antibody produced in mouse

~1 mg/mL, clone C5, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Mi

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Gene Regulation, Antibodies to Common Transcription Factors, Antibodies to Transcription Factors,
species reactivity   human, mouse, rat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunoprecipitation: suitable using 2 μg/mg protein lysate
  western blot: 1:500 using 10 μg of Mouse brain lysates
clone   C5, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 52-56 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MITF(4286)
mouse ... Mitf(17342)
rat ... Mitf(25094)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

N-terminal fragment of human microphthalmia protein.

General description

Microphthalmia (Mi in mouse or MITF in human) is a basic helix-loop-helix-leucine zipper (BHLH–ZIP) transcription factor involved in the differentiation, development and survival of melanocytes and cells of the retinal pigment epithelium, i.e. cells responsible for hair, skin, and eye color. It activates the expression of the melanocyte specific genes tyrosinase and TRP1 (tyrosinase-related protein 1) by binding as a homo- or heterodimer to a symmetrical DNA sequence (E box) (5′-CATGTG-3′) located within the M box found in their promoters. Microphthalmia also appears to be involved in the differentiation of mast cells, osteoclasts, basophils and natural killer cells.
Microphthalmia is expressed in a limited number of cell types including heart, mast cells, osteoclast precursors, and melanocytes. There are a number of different isoforms of microphthalmia resulting from alternative splicing and alternative promotors. These isoforms differ at their amino-termini and in their expression patterns.

Mouse monoclonal clone C5 anti-Microphthalmia antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia from human, mouse or rat.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal clone C5 anti-Microphthalmia antibody is used to tag serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia for detection and quantitation by techniques such as immunoblotting (doublet of 52-56 kDa), immunoprecipitation, immunohistochemistry on formalin-fixed paraffin embedded or frozen tissue sections, and gel shift. It is used as a probe to determine the roles of microphthalmia in the differentiation, development and survival of melanocytes and cells of the retinal pigment epithelium.

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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