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  • M6552 - Monoclonal Anti-Matrix Metalloproteinase-3 antibody produced in mouse

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M6552 Sigma

Monoclonal Anti-Matrix Metalloproteinase-3 antibody produced in mouse

clone SL-1 IIIC4, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-MMP-3

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix, Antibodies to Extracellular Matrix/Cytoskeleton,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using heat/pressure cooker pre-treatment
  immunohistochemistry (frozen sections): 1:50-1:100
  immunoprecipitation: 10 using protein lysate
  indirect immunofluorescence: 4-5 μg/mL
  western blot: 1:200-1:400 using conditioned medium from PMA-treated HT-1080 cells or bladder, breast, or ovarian carcinoma
clone   SL-1 IIIC4, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~48 kDa (active form)
  antigen mol wt ~57 kDa (latent form)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MMP3(4314)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

APMA-activated human matrix metalloproteinase-3 (stromelysin-1).

Physical form

Solution in 10 mM phosphate buffered saline and 0.2% bovine serum albumin, pH 7.4, containing 0.1% sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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HPA007875 Anti-MMP3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

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