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M6899 Sigma

Murashige and Skoog Basal Salts with minimal organics

powder, plant cell culture tested

Synonym: MSMO

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Properties

Related Categories Culture Media, Salts and Vitamin Mixes, Molecular Biology, Plant Biotechnology, Plant Tissue Culture
form   powder
suitability   plant cell culture tested
Featured Industry   Agriculture
storage temp.   2-8°C

Description

Formula variant

With the macro- and micronutrients, and vitamins as described by Linsmaier and Skoog (1965).

Media Formulation

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Shoot Multiplication Medium A (M6899 is a variant media) with macro- and micronutrients, vitamins as described by Linsmaier and Skoog.

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 1477 5.1 / PGII
WGK Germany 
1
Protocols & Articles

Protocols

Murashige Skoog - Plant Tissue Culture Protocol

M5524 – Murashige and Skoog basal salt mixture (MS) M0654 – Murashige and Skoog basal salt macronutrient solution M0529 – Murashige and Skoog basal salt micronutrient solution M2909 – Murashige and S...
Keywords: Mass spectrometry, Vitamins

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Peer-Reviewed Papers
15

References

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