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M6908 Sigma

Myeloperoxidase from human leukocytes

lyophilized powder, ≥50 units/mg protein

Synonym: MPO, Peroxidase, myelo

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Description

Analysis Note

Source material tested and found negative for antibody to HIV and for HBSAG.

Biochem/physiol Actions

Catalyzes oxidations by hydrogen peroxide, including cytotoxic actions on bacteria, fungi, and tumor cells. Also catalyzes cross-linking of immune complexes and inactivates chemotactic factors.

Other Notes

Lysosomal heme-containing protein.

Unit Definition

One unit will produce an increase in absorbance (A470) of 1.0 per min at pH 7.0 and 25 °C, calculated from the initial rate of reaction using guaiacol as substrate. Total reaction volume: 3.035 mL.

Physical form

Lyophilized from 50 mM sodium acetate buffer, pH 6.0, 0.1 M sodium chloride

Price and Availability

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Metabolomic Pathways Chart

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Anti-MPO antibody produced in rabbit

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Mouse MPO ELISA Kit

for plasma and cell culture supernatant

MPO Colorimetric Activity Assay Kit

sufficient for 100 colorimetric tests

Hanker-Yates substrate kit

Safety & Documentation

Safety Information

WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

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Peer-Reviewed Papers

References

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Epitope specificity determines pathogenicity and detectability in ANCA-associated vasculitis. Roth AJ, Ooi JD, Hess JJ, et al. J. Clin. Invest. 123(4), 1773-83, (2013)

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Serum phospholipid fatty acids, genetic variation in myeloperoxidase, and prostate cancer risk in heavy smokers: a gene-nutrient interaction in the carotene and retinol efficacy trial. Cheng TY, King IB, Barnett MJ, et al. Am. J. Epidemiol. 177(10), 1106-17, (2013)

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TRPV1 and TRPA1 antagonists prevent the transition of acute to chronic inflammation and pain in chronic pancreatitis. Schwartz ES, La JH, Scheff NN, et al. J. Neurosci. 33(13), 5603-11, (2013)

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Turnover capacity of coprinus cinereus peroxidase for phenol and monosubstituted phenols Aitken MD and Heck PE Biotechnol. Prog. 14(3), 487-92, (1998)

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Maltose-binding protein switches programmed cell death in Nicotiana glutinosa leaf cells. Gholizadeh A Tsitol. Genet. 48(2), 20-7, (2014)

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[NETs in pathogenesis of vasculitis]. Nakazawa D, Nishio S, Tomaru U, et al. Nihon Jinzo Gakkai Shi 56(2), 117-23, (2014)

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[Plasma levels of anti-oxidant markers during general anesthesia--a comparison between remifentanil- and epidural-based anesthesia]. Enohata K, Hasegawa-Moriyama M, Kuniyoshi T, et al. Masui. 63(3), 328-32, (2014)

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Relation of kynurenine/tryptophan with immune and inflammatory markers in coronary artery disease. Ozkan Y, Sukuroglu MK, Tulmac M, et al. Clin. Lab. 60(3), 391-6, (2014)

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Myeloperoxidase-mediated dysfunctional high-density lipoprotein. Getz GS and Reardon CA Arterioscler. Thromb. Vasc. Biol. 34(4), 695-6, (2014)

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[Homocysteine peroxidase activity in rat blood plasma: stoichiometry and enzymatic character of the reaction]. Razygraev AV Biomed. Khim. 59(6), 636-43, (2013)

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Biosynthesis of N,N-dimethyltryptamine (DMT) in a melanoma cell line and its metabolization by peroxidases. Gomes MM, Coimbra JB, Clara RO, et al. Biochem. Pharmacol. 88(3), 393-401, (2014)

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Protective effect of montelukast, a cysteinyl leukotriene receptor-1 antagonist, against intestinal ischemia-reperfusion injury in the rat. Duran A, Otiük H, Terzi EH, et al. Acta Chir. Belg. 113(6), 401-5, (2013)

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[Inner ear 3D-FLAIR magnetic resonance image evaluation of MPO-ANCA related angitis patients]. Kato K, Sone M, Teranishi M, et al. Nippon. Jibiinkoka Gakkai Kaiho 116(11), 1192-9, (2013)

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Resolvin D1 attenuates lipopolysaccharide induced acute lung injury through CXCL-12/CXCR4 pathway. Yaxin W, Shanglong Y, Huaqing S, et al. J. Surg. Res. 188(1), 213-21, (2014)

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[Effect of population density on enzymatic activity of antioxidative and phenol oxidase systems of imagoes and nymphs of the marble cockroach Nauphoeta cinerea]. Murzagulov GS, Saltykova ES, Gaĭfullina LR, et al. Zh. Evol. Biokhim. Fiziol. 49(1), 24-9, (2013)

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Acute aortocaval fistula: role of low perfusion pressure and subendocardial remodeling on left ventricular function. Mazzo FR, de Carvalho Frimm C, Moretti AI, et al. Int. J. Exp. Pathol. 94(3), 178-87, (2013)

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Design, synthesis, and structure-activity relationship studies of novel 3-alkylindole derivatives as selective and highly potent myeloperoxidase inhibitors. Soubhye J, Aldib I, Elfving B, et al. J. Med. Chem. 56(10), 3943-58, (2013)

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β2 integrin-mediated cell-cell contact transfers active myeloperoxidase from neutrophils to endothelial cells. Jerke U, Rolle S, Purfürst B, et al. J. Biol. Chem. 288(18), 12910-9, (2013)

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Ocular inflammation and corneal permeability alteration by benzalkonium chloride in rats: a protective effect of a myosin light chain kinase inhibitor. Droy-Lefaix MT, Bueno L, Caron P, et al. Invest. Ophthalmol. Vis. Sci. 54(4), 2705-10, (2013)

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Mammalian target of rapamycin regulates IL-10 and resistance to Pseudomonas aeruginosa corneal infection. Foldenauer ME, McClellan SA, Berger EA, et al. J. Immunol. 190(11), 5649-58, (2013)

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Berberine attenuates bleomycin induced pulmonary toxicity and fibrosis via suppressing NF-κB dependant TGF-β activation: a biphasic experimental study. Chitra P, Saiprasad G, Manikandan R, et al. Toxicol. Lett. 219(2), 178-93, (2013)

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Nrf2 is not required for epithelial prohibitin-dependent attenuation of experimental colitis. Kathiria AS, Butcher MA, Hansen JM, et al. Am. J. Physiol. Gastrointest. Liver Physiol. 304(10), G885-96, (2013)

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The myeloperoxidase product hypochlorous acid generates irreversible high-density lipoprotein receptor inhibitors. Binder V, Ljubojevic S, Haybaeck J, et al. Arterioscler. Thromb. Vasc. Biol. 33(5), 1020-7, (2013)

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Oxidation-reduction properties of compounds I and II of Arthromyces ramosus peroxidase. Farhangrazi ZS, Copeland BR, Nakayama T, et al. Biochemistry 33(18), 5647-52, (1994)

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Ethyl pyruvate significantly inhibits tumour necrosis factor-α, interleukin-1β and high mobility group box 1 releasing and attenuates sodium taurocholate-induced severe acute pancreatitis associated with acute lung injury. Luan ZG, Zhang J, Yin XH, et al. Clin. Exp. Immunol. 172(3), 417-26, (2013)

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Chemotactic factor inactivation by stimulated human neutrophils mediated by myeloperoxidase-catalyzed methionine oxidation. Clark, R.A., and Szot, S. J. Immunol. 128, 1507-1513, (1982)

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Covalent cross-linking of immune complexes by oxygen radicals and nitrite. Uesugi, M., et al. J. Immunol. 161, 1422-1427, (1998)

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A novel benzo[d]imidazole derivate prevents the development of dextran sulfate sodium-induced murine experimental colitis via inhibition of NLRP3 inflammasome. Liu W, Guo W, Wu J, et al. Biochem. Pharmacol. 85(10), 1504-12, (2013)

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Expression profiling of four defense-related buffalograss transcripts in response to chinch bug (Hemiptera: Blissidae) feeding. Ramm C, Saathoff A, Donze T, et al. J. Econ. Entomol. 106(6), 2568-76, (2013)

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An abundant dysfunctional apolipoprotein A1 in human atheroma. Huang Y, DiDonato JA, Levison BS, et al. Nat. Med. 20(2), 193-203, (2014)

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Effects of native and myeloperoxidase-modified apolipoprotein a-I on reverse cholesterol transport and atherosclerosis in mice. Hewing B, Parathath S, Barrett T, et al. Arterioscler. Thromb. Vasc. Biol. 34(4), 779-89, (2014)

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Peroxidase-H2O-halide system: Cytotoxic effect on mammalian tumor cells. Clark, R.A., et al. Blood 45, 161, (1975)

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Assessment of myeloperoxidase activity by the conversion of hydroethidine to 2-chloroethidium. Maghzal GJ, Cergol KM, Shengule SR, et al. J. Biol. Chem. 289(9), 5580-95, (2014)

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Chrysin ameliorates chemically induced colitis in the mouse through modulation of a PXR/NF-κB signaling pathway. Dou W, Zhang J, Zhang E, et al. J. Pharmacol. Exp. Ther. 345(3), 473-82, (2013)

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Polyandric acid A, a clerodane diterpenoid from the Australian medicinal plant Dodonaea polyandra, attenuates pro-inflammatory cytokine secretion in vitro and in vivo. Simpson BS, Luo X, Costabile M, et al. J. Nat. Prod. 77(1), 85-91, (2014)

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Oxidative cross-linking of immune complexes by human polymorphonuclear leukocytes. Jasin, H.E. J. Clin. Invest. 81, 6-15, (1988)

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Matrix metalloproteinase inhibition lowers mortality and brain injury in experimental pneumococcal meningitis. Liechti FD, Grandgirard D, Leppert D, et al. Infect. Immun. 82(4), 1710-8, (2014)

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Evaluation of circulating levels of inflammatory and bone formation markers in axial spondyloarthritis. de Andrade KR, de Castro GR, Vicente G, et al. Int. Immunopharmacol. 21(2), 481-6, (2014)

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The bactericidal effects of the respiratory burst and the myeloperoxidase system isolated in neutrophil cytoplasts. Odell, E.W., and Segal, A.W. Biochim. Biophys. Acta 97, 266-274, (1988)

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Butyrate protects liver against ischemia reperfusion injury by inhibiting nuclear factor kappa B activation in Kupffer cells. Qiao YL, Qian JM, Wang FR, et al. J. Surg. Res. 187(2), 653-9, (2014)

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Dose- and time-dependent effects of lipopolysaccharide on technetium-99-m-labeled diethylene-triamine pentaacetatic acid clearance, respiratory system mechanics and pulmonary inflammation. Kastis GA, Toumpanakis D, Loverdos K, et al. Exp. Biol. Med. (Maywood.) 238(2), 209-22, (2013)

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Differences in the behavior of advanced glycation end products and advanced oxidation protein products in patients with allergic rhinitis. Di Lorenzo G, Minciullo PL, Leto-Barone MS, et al. J. Investig. Allergol. Clin. Immunol. 23(2), 101-6, (2013)

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Prussian blue modified ferritin as peroxidase mimetics and its applications in biological detection. Zhang W, Zhang Y, Chen Y, et al. J. Nanosci. Nanotechnol. 13(1), 60-7, (2013)

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Absence of NF-κB subunit p50 ameliorates cold immobilization stress-induced gastric ulcers. Ye B, Zhou PY, Jia M, et al. Biochem. Biophys. Res. Commun. 434(3), 547-51, (2013)

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Oxidative stress and inflammation in lean and obese subjects with polycystic ovary syndrome. Blair SA, Kyaw-Tun T, Young IS, et al. J. Reprod. Med. 58(3-4), 107-14, (2013)

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Myeloperoxidase G-463A polymorphism and susceptibility to coronary artery disease: a meta-analysis. Tang N, Wang Y, and Mei Q Gene 523(2), 152-7, (2013)

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Immunopathologic co-localization of MPO, IgG, and C3 in glomeruli in human MPO-ANCA-associated glomerulonephritis. Kawashima S, Arimura Y, Sano K, et al. Clin. Nephrol. 79(4), 292-301, (2013)

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Copper induced oxidative stress in tea (Camellia sinensis) leaves. Saha D, Mandal S, and Saha A J. Environ Biol. 33(5), 861-6, (2012)

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Differential antioxidative response of tolerant and sensitive maize (Zea mays L.) genotypes to drought stress at reproductive stage. Chugh V, Kaur N, Grewal MS, et al. Indian J. Biochem. Biophys. 50(2), 150-8, (2013)

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Leukocyte activation markers and oxidative status in chronic kidney disease. Carollo C, Lo Presti R, and Caimi G Minerva Urol. Nefrol. 65(1), 69-76, (2013)

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Myeloperoxidase and progression of aortic valve stenosis in patients undergoing hemodialysis. Wada S, Sugioka K, Naruko T, et al. J. Heart Valve Dis. 22(5), 640-7, (2013)

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Effect of myeloperoxidase inhibition on gene expression profiles in HL-60 cells exposed to 1,2,4,-benzenetriol. Miyahara E, Nishikawa T, Takeuchi T, et al. Toxicology 317, 50-7, (2014)

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Oxidative potential of ultraviolet-A irradiated or nonirradiated suspensions of titanium dioxide or silicon dioxide nanoparticles on Allium cepa roots. Koce JD, Drobne D, Klančnik K, et al. Environ. Toxicol. Chem. 33(4), 858-67, (2014)

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Histone deacetylase inhibitors trichostatin A and suberoylanilide hydroxamic acid attenuate ventilator-induced lung injury. Chen HY, Li L, and Fu ZJ Pharmazie 69(1), 55-9, (2014)

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Leucomycin A3, a 16-membered macrolide antibiotic, inhibits influenza A virus infection and disease progression. Sugamata R, Sugawara A, Nagao T, et al. J. Antibiot. 67(3), 213-22, (2014)

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Biomarkers of cardiovascular disease are increased in untreated chronic periodontitis: a case control study. Ramírez JH, Parra B, Gutierrez S, et al. Aust. Dent. J. 59(1), 29-36, (2014)

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Inhibition of NADPH oxidase prevents acute lung injury in obese rats following severe trauma. Xiang L, Lu S, Mittwede PN, et al. Am. J. Physiol. Heart Circ. Physiol. 306(5), H684-9, (2014)

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Sunscreen protection against ultraviolet-induced oxidative stress: evaluation of reduced glutathione levels, metalloproteinase secretion, and myeloperoxidase activity. Vilela FM, Fonseca YM, Vicentini FT, et al. Pharmazie 68(11), 872-6, (2013)

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Peroxidases from wheat germ. TAGAWA K, SHIN M, and OKUNUKI K Nature 183(4654), 111, (1959)

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The isolation and purification of lactoperoxidase by ion exchange chromatography. MORRISON M, HAMILTON HB, and STOTZ E J. Biol. Chem. 228(2), 767-76, (1957)

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A simple method for the preparation of horseradish peroxidase. KENTEN RH and MANN PJ Biochem. J. 57(2), 347-8, (1954)

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The fungicidal mechanisms of human monocytes. I. Evidence for myeloperoxidase-linked and myeloperoxidase-independent candidacidal mechanisms. Lehrer, R.I. J. Clin. Invest. 55, 338-346, (1975)

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