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M7027 Sigma

Methyl cellulose

viscosity: 15 cP, BioReagent, suitable for cell culture

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Properties

Related Categories Cell Culture, Miscellaneous Reagents and Supplements, Reagents and Supplements More...
product line   BioReagent
viscosity   12-18 cP, 2 % in H2O(20 °C)
suitability   suitable for cell culture

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Use to create semi-solid matrices, as a cell culture gel, for culture of colony forming cells such as CFU-G; CFU-GM; cells and other cells used in toxicity assays. Low viscosity, not for plaquing assays or cloning.

Packaging

100, 250 g in poly bottle

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How can I make a solution of Methyl cellulose, Product M7027?
There are two methods for solubilizing methylcellulose. Please see the product insert for Product No. M0387 for the protocols. http://www.sigmaaldrich.com/sigma/product%20information%20sheet/m0387pis.pdf
Can I use this Methyl cellulose, Product M7027, for cloning or plaque assays?
Probably not. Product No. M7027 has a very low viscosity (viscosity 15 cP, 2% solution in water) and is used for thickening culture media.  Product No. M0387 (viscosity 1,500 cP, 2% solution in water) is recommended for plaque assays, and Product No. M0512 (viscosity 4,000 cP, 2% solution in water) is recommended for cloning or colony growth assays.
How do I sterilize a solution of Methyl cellulose, Product M7027?
A 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to chunks of methylcellulose falling out of solution.
What concentration of Methyl cellulose, Product M7027, is used for cell culture?
Product No. M7027 is used at approximately 50 g/L in media to enhance viscosity of culture media.
Is Methyl cellulose, Product M7027, the same as Methocel?
Methocel is a brand name and is a synonym for methylcellulose.
How do I store a stock solution of Methyl cellulose, Product M7027?
A sterile solution of methylcellulose can be stored at room temperature for up to 1 year.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles
Peer-Reviewed Papers
15

References

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