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M7154 Sigma

2-Mercaptoethanol

for electrophoresis

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

Purchase

Properties

Related Categories Core Bioreagents, General Reagents, Life Science Reagents for Northern and Southern Blotting, Life Science Reagents for Protein Electrophoresis, Life Science Reagents for Western Blotting,
grade   for electrophoresis
vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
expl. lim.   18 %
concentration   14.3 M (pure liquid)
refractive index   n20/D 1.500(lit.)
bp   157 °C(lit.)
density   1.114 g/mL at 25 °C(lit.)
cation traces   Cu: <1 ppm
  Fe: <1 ppm
  Pb: <5 ppm
  Zn: <1 ppm

Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

This product has been replaced by M3148-SIGMA | 2-Mercaptoethanol for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Price and Availability

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Safety & Documentation

Safety Information

Signal word 
Danger
Target organs 
Heart, Liver
RIDADR 
UN 2966 6.1 / PGII
WGK Germany 
3
RTECS 
KL5600000
Flash Point(F) 
165.2 °F
Flash Point(C) 
74 °C

Protocols & Articles

Articles

A functional assay for microRNA target identification and validation

MicroRNAs (miRNA) are a class of small RNA molecules that regulate numerous critical cellular processes and bind to partially complementary sequences resulting in down-regulation of their target gene...
Joop Gäken, Azim M. Mohamedali, Jie Jiang, Farooq Malik, Doris Stangl, Alexander E. Smith, Constantinos Chronis, Austin G. Kulasekararaj, Nicolas S. B. Thomas, Farzin farzaneh, Mahavash Tavassoli, and Ghulam J. Maufi
Nucleic Acids Research 2012, 1-9
Keywords: Cellular processes, Sequences, Western blot

Peer-Reviewed Papers

References

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Stromagenesis During Tumorigenesis: Characterization Of Tumor-associated Fibroblasts And Stroma-derived 3D Matrices. Castelló-Cros, R., and Cukierman, E. Methods Mol. Biol. 522, 275-305, (2009)

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A method for nanofluidic device prototyping using elastomeric collapse. Seung-min Park et al Proc. Natl. Acad. Sci. U. S. A. 106, 15549-54, (2009)

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Efficacy of liposomal bismuth-ethanedithiol-loaded tobramycin after intratracheal administration in rats with pulmonary Pseudomonas aeruginosa infection. Alhariri M and Omri A Antimicrob. Agents Chemother. 57(1), 569-78, (2013)

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Mercaptoethanol capped CdSe quantum dots and CdSe/ZnS core/shell: synthesis, characterization and cytotoxicity evaluation. Painuly D, Bhatt A, and Krishnan VK J. Biomed. Nanotechnol. 9(2), 257-66, (2013)

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On the surface interactions of proteins with lignin. Salas C, Rojas OJ, Lucia LA, et al. ACS Appl. Mater. Interfaces 5(1), 199-206, (2013)

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A microplate fluorescence assay for DAPA aminotransferase by detection of the vicinal diamine 7,8-diaminopelargonic acid. Mann S, Eveleigh L, Lequin O, et al. Anal. Biochem. 432(2), 90-6, (2013)

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Scalable culture and cryopreservation of human embryonic stem cells on microcarriers. Ying Nie et al Biotechnol. Prog. 25, 20-31, (2009)

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The relative efficiency of homology-directed repair has distinct effects on proper anaphase chromosome separation. Laulier C Nucleic Acids Res. 39(14), 5935-44, (2011)

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Pro-inflammatory cytokine/chemokine production by reovirus treated melanoma cells is PKR/NF-κB mediated and supports innate and adaptive anti-tumour immune priming. Steele, L Mol. Cancer 10, 20, (2011)

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Cyclin-dependent kinase 5 promotes pancreatic β-cell survival via Fak-Akt signaling pathways. Daval M Diabetes 60(4), 1186-97, (2011)

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Integrative DNA methylation and gene expression analyses identify DNA packaging and epigenetic regulatory genes associated with low motility sperm. Pacheco SE PLoS ONE 6(6), e20280, (2011)

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AGEs decrease insulin synthesis in pancreatic β-cell by repressing Pdx-1 protein expression at the post-translational level. Shu T, Zhu Y, Wang H, et al. PLoS ONE 6(4), e18782, (2011)

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Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice. Jinhong Meng et al PLoS ONE 6, e17454, (2011)

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Carbon monoxide blocks lipopolysaccharide-induced gene expression by interfering with proximal TLR4 to NF-kappaB signal transduction in human monocytes. Chhikara M PLoS ONE 4(12), e8139, (2009)

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Endothelial cells in co-culture enhance embryonic stem cell differentiation to pancreatic progenitors and insulin-producing cells through BMP signaling. Talavera-Adame D, Wu G, He Y, et al. Stem Cell Rev. 7(3), 532-43, (2011)

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Administration of M. leprae Hsp65 interferes with the murine lupus progression. Marengo EB, de Moraes LV, Faria M, et al. PLoS ONE 3(8), e3025, (2008)

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Impaired fertilizing ability of superoxide dismutase 1-deficient mouse sperm during in vitro fertilization. Tsunoda S, Kawano N, Miyado K, et al. Biol. Reprod. 87(5), 121, (2012)

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Characterization of RimO, a new member of the methylthiotransferase subclass of the radical SAM superfamily. Lee KH, et al. Biochemistry 48(42), 10162-74, (2009)

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In vitro neuronal induction of adipose-derived stem cells and their fate after transplantation into injured mouse brain. Pavlova G, Lopatina T, Kalinina N, et al. Curr. Med. Chem. 19(30), 5170-7, (2012)

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Toll-like receptor 2 dependent immunogenicity of glycoconjugate vaccines containing chemically derived zwitterionic polysaccharides. Gallorini S Proc. Natl. Acad. Sci. U. S. A. 106(41), 17481-6, (2009)

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The influences of reaction conditions on magnetic porous pseudo-carbon paste electrode for schistosoma egg antigen detection. Xu L, Du J, and He N J. Nanosci. Nanotechnol. 13(3), 2302-6, (2013)

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Rapid formal hydrolysis of peptide-αthioesters. Gates ZP, Stephan JR, Lee DJ, et al. Chem. Commun. (Camb.) 49(8), 786-8, (2013)

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Structure of a eukaryotic thiaminase I. Kreinbring CA, Remillard SP, Hubbard P, et al. Proc. Natl. Acad. Sci. U. S. A. 111(1), 137-42, (2014)

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The protein interactions and rheological properties of skim milk heated in the presence of low levels of reducing agent. Nguyen NH, Wong M, Havea P, et al. Food Chem. 138(2-3), 1604-9, (2013)

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Activation of T-cells by a macrophage or 2-mercaptoethanol activated serum factor is essential for induction of a primary immune response to heterologous red cells in vitro. Opitz HG, Lemke H, and Hewlett G Immunol. Rev. 40, 53-77, (1978)

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BNP signaling is crucial for embryonic stem cell proliferation. Abdelalim EM PLoS ONE 4(4), e5341, (2009)

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Reovirus nonstructural protein sigma1s is required for establishment of viremia and systemic dissemination. Boehme KW Proc. Natl. Acad. Sci. U. S. A. 106(47), 19986-91, (2009)

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Microsomal prostaglandin E synthase type 2 (mPGES2) is a glutathione-dependent heme protein, and dithiothreitol dissociates the bound heme to produce active prostaglandin E2 synthase in vitro. Takusagawa F J. Biol. Chem. 288(14), 10166-75, (2013)

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Cigarette smoke extract (CSE) delays NOD2 expression and affects NOD2/RIPK2 interactions in intestinal epithelial cells. Aldhous MC, Soo K, Stark LA, et al. PLoS ONE 6(9), e24715, (2011)

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Reconstitution of Schwannian stroma in neuroblastomas using human bone marrow stromal cells. Wenlin Du et al Am. J. Pathol. 173, 1153-64, (2008)

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Regulators of cellular levels of histone acetylation in Saccharomyces cerevisiae. Peng W, Togawa C, Zhang K, et al. Genetics 179(1), 277-89, (2008)

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Multifunctional thiols from the highly selective reaction of mercaptoalcohols with chlorosilanes. Jennings AR and Son DY Chem. Commun. (Camb.) 49(33), 3467-9, (2013)

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Human augmenter of liver regeneration: probing the catalytic mechanism of a flavin-dependent sulfhydryl oxidase. Schaefer-Ramadan S, Gannon SA, and Thorpe C Biochemistry 52(46), 8323-32, (2013)

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Differences in Salmonella enterica serovar Typhimurium strain invasiveness are associated with heterogeneity in SPI-1 gene expression. Leann Clark et al Microbiology 157, 2072-83, (2011)

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Beta-mecaptoethanol suppresses inflammation and induces adipogenic differentiation in 3T3-F442A murine preadipocytes. Guo W, Li Y, Liang W, et al. PLoS ONE 7(7), e40958, (2012)

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Gene expression analysis of embryonic stem cells expressing VE-cadherin (CD144) during endothelial differentiation. Nikolova-Krstevski V, Bhasin M, Otu HH, et al. BMC Genomics 9, 240, (2008)

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A point mutation produced a class 3 aldehyde dehydrogenase with increased protective ability against the killing effect of cyclophosphamide. Ho KK Biochem. Pharmacol. 76(5), 690-6, (2008)

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Carbonic anhydrase inhibitors: the very weak inhibitors dithiothreitol, beta-mercaptoethanol, tris(carboxyethyl)phosphine and threitol interfere with the binding of sulfonamides to isozymes II and IX. Alessio Innocenti et al Bioorg. Med. Chem. Lett. 18, 1898-903, (2008)

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Bradykinin-stimulated cyclooxygenase activity stimulates vas deferens epithelial anion secretion in vitro in swine and humans. Fernando Pierucci-Alves et al Biol. Reprod. 79, 501-9, (2008)

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Oxygen tension modulates differentiation and primary macrophage functions in the human monocytic THP-1 cell line. Grodzki AC, Giulivi C, and Lein PJ PLoS ONE 8(1), e54926, (2013)

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Interaction of fish aryl hydrocarbon receptor paralogs (AHR1 and AHR2) with the retinoblastoma protein. Merson RR Aquat. Toxicol. 94(1), 47-55, (2009)

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Oxidative folding of lysozyme with aromatic dithiols, and aliphatic and aromatic monothiols. Amar S Patel et al Bioorg. Med. Chem. 20, 1020-8, (2012)

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Transforming growth factor-β-inducible early response gene 1 is a novel substrate for atypical protein kinase Cs. Alemu, E.A., et al. Cell. Mol. Life Sci. 68, 1953-68, (2011)

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The ATP/DNA ratio is a better indicator of islet cell viability than the ADP/ATP ratio. T.M. Suszynski et al Transplant. Proc. 40, 346-50, (2009)

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Differentiation of human adipose-derived mesenchymal stem cell into insulin-producing cells: an in vitro study. Moshtagh PR, Emami SH, and Sharifi AM J. Physiol. Biochem. 69(3), 451-8, (2013)

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Pathogenic MOG-reactive CD8+ T cells require MOG-reactive CD4+ T cells for sustained CNS inflammation during chronic EAE. Bettini M J. Neuroimmunol. 213(1-2), 60-8, (2009)

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A protocol for stripping and reprobing of Western blots originally developed with colorimetric substrate TMB. Kar P, Agnihotri SK, Sharma A, et al. Electrophoresis 33(19-20), 3062-5, (2012)

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Improved performance of nanowire-quantum-dot-polymer solar cells by chemical treatment of the quantum dot with ligand and solvent materials. Nadarajah A, Smith T, and Könenkamp R Nanotechnology 23(48), 485403, (2012)

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Expression of RHOGTPase regulators in human myometrium. O'Brien M Reprod. Biol. Endocrinol. 6, 1, (2008)

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Caveolin-1 regulates neural differentiation of rat bone mesenchymal stem cells into neurons by modulating Notch signaling. Wang S, Kan Q, Sun Y, et al. Int. J. Dev. Neurosci. 31(1), 30-5, (2013)

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Use of long-term cultured embryoid bodies may enhance cardiomyocyte differentiation by BMP2. Kim YY Yonsei Med. J. 49(5), 819-27, (2008)

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Insulin related compounds and identification. Min CK, Lee JW, Kim CK, et al. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 908, 105-12, (2012)

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A β-mercaptoethanol-modified enzyme-linked immunosorbent assay for diagnosis of canine visceral leishmaniasis. Barral-Veloso L, Semião-Santos SJ, de Andrade PP, et al. J. Vet. Diagn. Invest. 25(2), 239-42, (2013)

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Racemization of the aspartic acid residue of amyloid-β peptide by a radical reaction. Tambo K, Yamaguchi T, Kobayashi K, et al. Biosci. Biotechnol. Biochem. 77(2), 416-8, (2013)

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Micro-emulsion synthesis, surface modification, and photophysical properties of Zn(1-x) Mn(x)S nanocrystals for biomolecular recognition. Mohagheghpour E, Moztarzadeh F, Rabiee M, et al. IEEE Trans. Nanobioscience 11(4), 317-23, (2012)

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The effects of bestatin on humoral response to sheep erythrocytes in non-treated and cyclophosphamide-immunocompromised mice. Lis M, Szczypka M, Suszko A, et al. Immunopharmacol. Immunotoxicol. 35(1), 133-8, (2013)

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Inhibition of cell division in mouse B-cell hybridomas: an overlooked property of 2-mercaptoethanol and its impact on in vitro antibody production. Abebe M, Dirieh Y, et al. Hybridoma 31, 240-245, (2012)

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Alteration of GI symptoms in a cow with Johne disease by the dietary organosulfur, 2-mercaptoethanol. Click RE Virulence 3(6), 543-5, (2012)

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Merck 14,5869

Beil. 1,IV,2428

Aldrich MSDS 1, 1162:C / Corp MSDS 1 (2), 2209:A / FT-IR 2 (1), 415:B / FT-IR 1 (1), 262:B / FT-NMR 1 (1), 422:B / IR-Spectra (2), 138:E / IR-Spectra (3), 154:F / NMR-Reference 2 (1), 228:B / RegBook 1 (1), 281:L / Sax 6, 1744 / Sigma FT-IR 1 (2), 700:C / Structure Index 1, 38:D:7 / Vapor Phase 3, 344:C

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