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M7154 Sigma

2-Mercaptoethanol

for electrophoresis

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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Properties

Related Categories Core Bioreagents, General Reagents, Life Science Reagents for Northern and Southern Blotting, Life Science Reagents for Protein Electrophoresis, Life Science Reagents for Western Blotting,
grade   for electrophoresis
vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
expl. lim.   18 %
concentration   14.3 M (pure liquid)
refractive index   n20/D 1.500(lit.)
bp   157 °C(lit.)
density   1.114 g/mL at 25 °C(lit.)
cation traces   Cu: <1 ppm
  Fe: <1 ppm
  Pb: <5 ppm
  Zn: <1 ppm

Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

This product has been replaced by M3148-SIGMA | 2-Mercaptoethanol for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Price and Availability

Safety & Documentation

Safety Information

Signal word 
Danger
Target organs 
Heart, Liver
RIDADR 
UN 2966 6.1 / PGII
WGK Germany 
3
RTECS 
KL5600000
Flash Point(F) 
165.2 °F
Flash Point(C) 
74 °C
Protocols & Articles

Articles

A functional assay for microRNA target identification and validation

MicroRNAs (miRNA) are a class of small RNA molecules that regulate numerous critical cellular processes and bind to partially complementary sequences resulting in down-regulation of their target gene...
Joop Gäken, Azim M. Mohamedali, Jie Jiang, Farooq Malik, Doris Stangl, Alexander E. Smith, Constantinos Chronis, Austin G. Kulasekararaj, Nicolas S. B. Thomas, Farzin farzaneh, Mahavash Tavassoli, and Ghulam J. Maufi
Nucleic Acids Research 2012, 1-9
Keywords: Cellular processes, Sequences, Western blot

Peer-Reviewed Papers
15

References

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