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M7154 Sigma

2-Mercaptoethanol

for electrophoresis

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

Properties

Related Categories Core Bioreagents, General Reagents, Life Science Reagents for Northern and Southern Blotting, Life Science Reagents for Protein Electrophoresis, Life Science Reagents for Western Blotting,
grade   for electrophoresis
vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
expl. lim.   18 %
concentration   14.3 M (pure liquid)
refractive index   n20/D 1.500(lit.)
bp   157 °C(lit.)
density   1.114 g/mL at 25 °C(lit.)
cation traces   Cu: <1 ppm
  Fe: <1 ppm
  Pb: <5 ppm
  Zn: <1 ppm

Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Price and Availability


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