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M7154 Sigma

2-Mercaptoethanol

for electrophoresis

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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Properties

Related Categories Core Bioreagents, General Reagents, Life Science Reagents for Northern and Southern Blotting, Life Science Reagents for Protein Electrophoresis, Life Science Reagents for Western Blotting,
grade   for electrophoresis
vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
expl. lim.   18 %
concentration   14.3 M (pure liquid)
refractive index   n20/D 1.500(lit.)
bp   157 °C(lit.)
density   1.114 g/mL at 25 °C(lit.)
cation traces   Cu: <1 ppm
  Fe: <1 ppm
  Pb: <5 ppm
  Zn: <1 ppm

Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

This product has been replaced by M3148-SIGMA | 2-Mercaptoethanol for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Price and Availability

TruPAGE™ Pre-cast Polyacrylamide Gels


TruPAGE™ Pre-cast Polyacrylamide GelsTruPAGE Gels run true every time!

  • . 2-Year Shelf Life
  • . Superior Band Resolution
  • . Strong & Tear Resistant
  • . Higher Capacity Wells



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Safety & Documentation

Safety Information

Signal word 
Danger
Target organs 
Heart, Liver
Hazard Codes (Europe) 
T,N
Risk Statements (Europe) 
Safety Statements (Europe) 
26-36/37/39-45-60-61
RIDADR 
UN 2966 6.1 / PGII
WGK Germany 
3
RTECS 
KL5600000
Flash Point(F) 
165.2 °F
Flash Point(C) 
74 °C

Protocols & Articles

Articles

A functional assay for microRNA target identification and validation

MicroRNAs (miRNA) are a class of small RNA molecules that regulate numerous critical cellular processes and bind to partially complementary sequences resulting in down-regulation of their target gene...
Joop Gäken, Azim M. Mohamedali, Jie Jiang, Farooq Malik, Doris Stangl, Alexander E. Smith, Constantinos Chronis, Austin G. Kulasekararaj, Nicolas S. B. Thomas, Farzin farzaneh, Mahavash Tavassoli, and Ghulam J. Maufi
Nucleic Acids Research 2012, 1-9
Keywords: Cellular processes, Sequences, Western blot

Peer-Reviewed Papers

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The ATP/DNA ratio is a better indicator of islet cell viability than the ADP/ATP ratio. T.M. Suszynski et al Transplant. Proc. 40, 346-50, (2009)

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A point mutation produced a class 3 aldehyde dehydrogenase with increased protective ability against the killing effect of cyclophosphamide. Ho KK Biochem. Pharmacol. 76(5), 690-6, (2008)

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Efficacy of liposomal bismuth-ethanedithiol-loaded tobramycin after intratracheal administration in rats with pulmonary Pseudomonas aeruginosa infection. Alhariri M and Omri A Antimicrob. Agents Chemother. 57(1), 569-78, (2013)

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Oxygen tension modulates differentiation and primary macrophage functions in the human monocytic THP-1 cell line. Grodzki AC, Giulivi C, and Lein PJ PLoS ONE 8(1), e54926, (2013)

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Pathogenic MOG-reactive CD8+ T cells require MOG-reactive CD4+ T cells for sustained CNS inflammation during chronic EAE. Bettini M J. Neuroimmunol. 213(1-2), 60-8, (2009)

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Endothelial cells in co-culture enhance embryonic stem cell differentiation to pancreatic progenitors and insulin-producing cells through BMP signaling. Talavera-Adame D, Wu G, He Y, et al. Stem Cell Rev. 7(3), 532-43, (2011)

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Carbonic anhydrase inhibitors: the very weak inhibitors dithiothreitol, beta-mercaptoethanol, tris(carboxyethyl)phosphine and threitol interfere with the binding of sulfonamides to isozymes II and IX. Alessio Innocenti et al Bioorg. Med. Chem. Lett. 18, 1898-903, (2008)

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Administration of M. leprae Hsp65 interferes with the murine lupus progression. Marengo EB, de Moraes LV, Faria M, et al. PLoS ONE 3(8), e3025, (2008)

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Inhibition of cell division in mouse B-cell hybridomas: an overlooked property of 2-mercaptoethanol and its impact on in vitro antibody production. Abebe M, Dirieh Y, et al. Hybridoma 31, 240-245, (2012)

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Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Laemmli, U.K. Nature 227, 680-685, (1970)

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Hames, B.D. and Rickwood, D. Gel Electrophoresis of Proteins: A Practical Approach 2nd ed., New York, NY , (1990), 32-35

Merck 14,5869

Beil. 1,IV,2428

Aldrich MSDS 1, 1162:C / Corp MSDS 1 (2), 2209:A / FT-IR 2 (1), 415:B / FT-IR 1 (1), 262:B / FT-NMR 1 (1), 422:B / IR-Spectra (2), 138:E / IR-Spectra (3), 154:F / NMR-Reference 2 (1), 228:B / RegBook 1 (1), 281:L / Sax 6, 1744 / Sigma FT-IR 1 (2), 700:C / Structure Index 1, 38:D:7 / Vapor Phase 3, 344:C

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