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M7279 Sigma

N,N′-Methylenebisacrylamide

powder, for molecular biology, for electrophoresis, ≥99.5%

Synonym: BIS

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Properties

Related Categories Acrylamides, Core Bioreagents, Electrophoresis of Glycans, Gel Cross-Linkers, Glycan Labeling and Analysis,
grade   for electrophoresis
  for molecular biology
assay   ≥99.5%
form   powder
impurities   ≤0.05% Free carboxylic acid (T)
  ≤50 mg/kg Total sulfur (as SO4 ) (ICP)
conductivity   ≤10 μmho (2% solution)
anion traces   chloride (Cl-): ≤50 mg/kg
cation traces   Ca: ≤10 ppm
  Cd: ≤5 ppm
  Co: ≤5 ppm
  Cr: ≤5 ppm
  Cu: ≤5 ppm
  Fe: ≤5 ppm
  K: ≤50 ppm
  Mg: ≤5 ppm
  Mn: ≤5 ppm
  Na: ≤50 ppm
  Ni: ≤5 ppm
  Pb: ≤5 ppm
  Zn: ≤5 ppm
absorption   A1%/290 ≤0.2
foreign activity   DNase and RNase, none detected

Description

Application

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

Packaging

25, 100, 250 g in poly bottle

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



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<I>N</I>,<I>N</I>′-Methylenebisacrylamide

suitable for electrophoresis (after filtration or allowing insolubles to settle)

<I>N</I>,<I>N</I>′-Methylenebisacrylamide solution

for electrophoresis, 2% in H2O

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
WGK Germany 
2
RTECS 
AS3678000

Protocols & Articles

Articles

NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field. This technique is non-destructive and can provide full structural info...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Cancer, Digestions, Electrophoresis, Nuclear magnetic resonance spectroscopy, Separation, Spectroscopy

Peer-Reviewed Papers

References

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Surface imprinted thin polymer film systems with selective recognition for bovine serum albumin. Kryscio DR and Peppas NA Anal. Chim. Acta 718, 109-15, (2012)

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Optical imaging. Expansion microscopy. Chen F, Tillberg PW, and Boyden ES Science 347(6221), 543-8, (2015)

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In situ growth of catalytic active Au-Pt bimetallic nanorods in thermoresponsive core-shell microgels. Lu Y, Yuan J, Polzer F, et al. ACS Nano 4(12), 7078-86, (2010)

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Synthesis of wheat straw cellulose-g-poly (potassium acrylate)/PVA semi-IPNs superabsorbent resin. Liu J, Li Q, Su Y, et al. Carbohydr. Polym. 94(1), 539-46, (2013)

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Preparation and characterization of molecularly imprinted polymer for di(2-ethylhexyl) phthalate: application to sample clean-up prior to gas chromatographic determination. Shaikh H, Memon N, Khan H, et al. J. Chromatogr. A 1247, 125-33, (2012)

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Double hydrogen-bonding pH-sensitive hydrogels retaining high-strengths over a wide pH range. Gao H, Wang N, Hu X, et al. Macromol. Rapid Commun. 34(1), 63-8, (2013)

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Preparation and characteristics of protein molecularly imprinted membranes on the surface of multiwalled carbon nanotubes. Zhang M, Huang J, Yu P, et al. Talanta 81(1-2), 162-6, (2010)

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Synthesis and characterization of carboxyl terminated poly(methacrylic acid) grafted chitosan/bentonite composite and its application for the recovery of uranium(VI) from aqueous media. Anirudhan TS and Rijith S J. Environ. Radioact. 106, 8-19, (2012)

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Sodium alginate/Na+-rectorite composite microspheres: preparation, characterization, and dye adsorption. Yang L, Ma X, and Guo N Carbohydr. Polym. 90(2), 853-8, (2012)

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Preparation and characterization of temperature-responsive poly(N-isopropylacrylamide-co-N,N'-methylenebisacrylamide) monolith for HPLC. Liu M, Liu H, Liu Y, et al. J. Chromatogr. A 1218(2), 286-92, (2011)

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Use of amidoximated hydrogel for removal and recovery of U(VI) ion from water samples. Hazer O and Kartal S Talanta 82(5), 1974-9, (2010)

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Multicolor polymer-dispersed liquid crystal. Kumano N, Seki T, Ishii M, et al. Adv. Mater. 23(7), 884-8, (2011)

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High throughput tryptic digestion via poly (acrylamide-co-methylenebisacrylamide) monolith based immobilized enzyme reactor. Wu S, Sun L, Ma J, et al. Talanta 83(5), 1748-53, (2011)

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High-strength hydrogel as a reusable adsorbent of copper ions. Wang N, Han Y, Liu Y, et al. J. Hazard. Mater. 213-214, 258-64, (2012)

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Development of an enzyme-free glucose sensor using the gate effect of a molecularly imprinted polymer. Yoshimi Y, Narimatsu A, Nakayama K, et al. J. Artif. Organs 12(4), 264-70, (2009)

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Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid. Milašinović N, Knežević-Jugović Z, Milosavljević N, et al. Int. J. Pharm. 436(1-2), 332-40, (2012)

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Direct transformation of N,N'-methylene bisacrylamide self-assembled fibers into polymer microtubes via RAFT polymerization. Li Q, Tang L, Xia Y, et al. Macromol. Rapid Commun. 34(2), 185-9, (2013)

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Novel poly(amidoamine)s with pendant primary amines as highly efficient gene delivery vectors. Liu M, Chen J, Cheng YP, et al. Macromol. Biosci. 10(4), 384-92, (2010)

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Selective recognition of organic pollutants in aqueous solutions with composite imprinted membranes. Kochkodan V, Hilal N, Melnik V, et al. Adv. Colloid Interface Sci. 159(2), 180-8, (2010)

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Preparation, characterization and application of a new stir bar sorptive extraction based on poly(vinylphthalimide-co-N,N'-methylenebisacrylamide) monolith. Huang X, Chen L, Yuan D, et al. J. Sep. Sci. 34(23), 3418-25, (2011)

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A one pot, one step method for the preparation of clickable hydrogels by photoinitiated polymerization. Yilmaz G, Kahveci MU, and Yagci Y Macromol. Rapid Commun. 32(23), 1906-9, (2011)

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Responsive behavior of tumor-marker-imprinted hydrogels using macromolecular cross-linkers. Miyata T, Hayashi T, Kuriu Y, et al. J. Mol. Recognit. 25(6), 336-43, (2012)

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Diffusion and binding of 5-fluorouracil in non-ionic hydrogels with interpolymer complexation. Zhou W, Lu P, Sun L, et al. Int. J. Pharm. 431(1-2), 53-60, (2012)

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In vivo evaluation of novel nanoparticles containing dexamethasone for ocular drug delivery on rabbit eye. Rafie F, Javadzadeh Y, Javadzadeh AR, et al. Curr. Eye Res. 35(12), 1081-9, (2010)

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Preparation and evaluation of a hydrophilic poly(2-hydroxyethyl methacrylate-co-N,N'-methylene bisacrylamide) monolithic column for pressurized capillary electrochromatography. Cheng J, Chen X, Cai Y, et al. Electrophoresis 34(8), 1189-96, (2013)

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Photocrosslinked bovine serum albumin hydrogels with partial retention of esterase activity. Abbate V, Kong X, and Bansal SS Enzyme Microb. Technol. 50(2), 130-6, (2012)

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Preparation, characterization and release of verapamil hydrochloride from polycaprolactone/acrylic acid (PCL/AA) hydrogels. Hanif M, Ranjha NM, Shoaib MH, et al. Pak. J. Pharm. Sci. 24(4), 503-11, (2011)

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Preliminary study of the relationship between water absorbency and zeta potentials of crosslinked poly(acrylic acid). Fan L, Zeng M, Zhou S, et al. J. Control. Release 152 Suppl 1, e260-2, (2011)

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Beil. 2,IV,1472

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