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  • M7279 - N,N′-Methylenebisacrylamide


M7279 Sigma


powder, for molecular biology, for electrophoresis, ≥99.5%

Synonym: BIS



Related Categories Acrylamides, Core Bioreagents, Electrophoresis of Glycans, Gel Cross-Linkers, Glycan Labeling and Analysis,
grade   for electrophoresis
  for molecular biology
assay   ≥99.5%
form   powder
impurities   ≤0.05% Free carboxylic acid (T)
  ≤50 mg/kg Total sulfur (as SO4 ) (ICP)
conductivity   ≤10 μmho (2% solution)
anion traces   chloride (Cl-): ≤50 mg/kg
cation traces   Ca: ≤10 ppm
  Cd: ≤5 ppm
  Co: ≤5 ppm
  Cr: ≤5 ppm
  Cu: ≤5 ppm
  Fe: ≤5 ppm
  K: ≤50 ppm
  Mg: ≤5 ppm
  Mn: ≤5 ppm
  Na: ≤50 ppm
  Ni: ≤5 ppm
  Pb: ≤5 ppm
  Zn: ≤5 ppm
absorption   A1%/290 ≤0.2
foreign activity   DNase and RNase, none detected



To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.


25, 100, 250 g in poly bottle

Price and Availability

Suggested Laboratory Gloves

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suitable for electrophoresis (after filtration or allowing insolubles to settle)

<I>N</I>,<I>N</I>′-Methylenebisacrylamide solution

for electrophoresis, 2% in H2O

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Personal Protective Equipment 
WGK Germany 

Protocols & Articles


NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field. This technique is non-destructive and can provide full structural info...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Cancer, Digestions, Electrophoresis, Nuclear magnetic resonance spectroscopy, Separation, Spectroscopy

Peer-Reviewed Papers


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Surface imprinted thin polymer film systems with selective recognition for bovine serum albumin. Kryscio DR and Peppas NA Anal. Chim. Acta 718, 109-15, (2012)


Optical imaging. Expansion microscopy. Chen F, Tillberg PW, and Boyden ES Science 347(6221), 543-8, (2015)


In situ growth of catalytic active Au-Pt bimetallic nanorods in thermoresponsive core-shell microgels. Lu Y, Yuan J, Polzer F, et al. ACS Nano 4(12), 7078-86, (2010)


Synthesis of wheat straw cellulose-g-poly (potassium acrylate)/PVA semi-IPNs superabsorbent resin. Liu J, Li Q, Su Y, et al. Carbohydr. Polym. 94(1), 539-46, (2013)


Preparation and characterization of molecularly imprinted polymer for di(2-ethylhexyl) phthalate: application to sample clean-up prior to gas chromatographic determination. Shaikh H, Memon N, Khan H, et al. J. Chromatogr. A 1247, 125-33, (2012)


Double hydrogen-bonding pH-sensitive hydrogels retaining high-strengths over a wide pH range. Gao H, Wang N, Hu X, et al. Macromol. Rapid Commun. 34(1), 63-8, (2013)


Preparation and characteristics of protein molecularly imprinted membranes on the surface of multiwalled carbon nanotubes. Zhang M, Huang J, Yu P, et al. Talanta 81(1-2), 162-6, (2010)


Synthesis and characterization of carboxyl terminated poly(methacrylic acid) grafted chitosan/bentonite composite and its application for the recovery of uranium(VI) from aqueous media. Anirudhan TS and Rijith S J. Environ. Radioact. 106, 8-19, (2012)


Sodium alginate/Na+-rectorite composite microspheres: preparation, characterization, and dye adsorption. Yang L, Ma X, and Guo N Carbohydr. Polym. 90(2), 853-8, (2012)


Preparation and characterization of temperature-responsive poly(N-isopropylacrylamide-co-N,N'-methylenebisacrylamide) monolith for HPLC. Liu M, Liu H, Liu Y, et al. J. Chromatogr. A 1218(2), 286-92, (2011)


Use of amidoximated hydrogel for removal and recovery of U(VI) ion from water samples. Hazer O and Kartal S Talanta 82(5), 1974-9, (2010)


Multicolor polymer-dispersed liquid crystal. Kumano N, Seki T, Ishii M, et al. Adv. Mater. 23(7), 884-8, (2011)


High throughput tryptic digestion via poly (acrylamide-co-methylenebisacrylamide) monolith based immobilized enzyme reactor. Wu S, Sun L, Ma J, et al. Talanta 83(5), 1748-53, (2011)


High-strength hydrogel as a reusable adsorbent of copper ions. Wang N, Han Y, Liu Y, et al. J. Hazard. Mater. 213-214, 258-64, (2012)


Development of an enzyme-free glucose sensor using the gate effect of a molecularly imprinted polymer. Yoshimi Y, Narimatsu A, Nakayama K, et al. J. Artif. Organs 12(4), 264-70, (2009)


Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid. Milašinović N, Knežević-Jugović Z, Milosavljević N, et al. Int. J. Pharm. 436(1-2), 332-40, (2012)


Direct transformation of N,N'-methylene bisacrylamide self-assembled fibers into polymer microtubes via RAFT polymerization. Li Q, Tang L, Xia Y, et al. Macromol. Rapid Commun. 34(2), 185-9, (2013)


Novel poly(amidoamine)s with pendant primary amines as highly efficient gene delivery vectors. Liu M, Chen J, Cheng YP, et al. Macromol. Biosci. 10(4), 384-92, (2010)


Selective recognition of organic pollutants in aqueous solutions with composite imprinted membranes. Kochkodan V, Hilal N, Melnik V, et al. Adv. Colloid Interface Sci. 159(2), 180-8, (2010)


Preparation, characterization and application of a new stir bar sorptive extraction based on poly(vinylphthalimide-co-N,N'-methylenebisacrylamide) monolith. Huang X, Chen L, Yuan D, et al. J. Sep. Sci. 34(23), 3418-25, (2011)


A one pot, one step method for the preparation of clickable hydrogels by photoinitiated polymerization. Yilmaz G, Kahveci MU, and Yagci Y Macromol. Rapid Commun. 32(23), 1906-9, (2011)


Responsive behavior of tumor-marker-imprinted hydrogels using macromolecular cross-linkers. Miyata T, Hayashi T, Kuriu Y, et al. J. Mol. Recognit. 25(6), 336-43, (2012)


Diffusion and binding of 5-fluorouracil in non-ionic hydrogels with interpolymer complexation. Zhou W, Lu P, Sun L, et al. Int. J. Pharm. 431(1-2), 53-60, (2012)


In vivo evaluation of novel nanoparticles containing dexamethasone for ocular drug delivery on rabbit eye. Rafie F, Javadzadeh Y, Javadzadeh AR, et al. Curr. Eye Res. 35(12), 1081-9, (2010)


Preparation and evaluation of a hydrophilic poly(2-hydroxyethyl methacrylate-co-N,N'-methylene bisacrylamide) monolithic column for pressurized capillary electrochromatography. Cheng J, Chen X, Cai Y, et al. Electrophoresis 34(8), 1189-96, (2013)


Photocrosslinked bovine serum albumin hydrogels with partial retention of esterase activity. Abbate V, Kong X, and Bansal SS Enzyme Microb. Technol. 50(2), 130-6, (2012)


Preparation, characterization and release of verapamil hydrochloride from polycaprolactone/acrylic acid (PCL/AA) hydrogels. Hanif M, Ranjha NM, Shoaib MH, et al. Pak. J. Pharm. Sci. 24(4), 503-11, (2011)


Preliminary study of the relationship between water absorbency and zeta potentials of crosslinked poly(acrylic acid). Fan L, Zeng M, Zhou S, et al. J. Control. Release 152 Suppl 1, e260-2, (2011)


Beil. 2,IV,1472

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