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M7383 Sigma

Malic Dehydrogenase from porcine heart

≥400 units/mg protein (biuret), ammonium sulfate suspension

Synonym: L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase

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Description

General description

Cytoplasmic Isozyme

Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Unit Definition

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4, 0.1 M KH2PO4, pH 7.0

Application

Malic dehydrogenase has been used in a study to assess the use of a long-wavelength homogeneous enzyme immunoassay for the determination of amikacin in water samples. It has also been used in a study to investigate the lipid metabolism in a liver exposed to cadmium.

Biochem/physiol Actions

The surfactant cetyl (hexadecyl) trimethylammonium bromide inhibits the cytoplasmic isoform of porcine heart malate dehydrogenase but has no effect on the mitochondrial form. SDS however; inhibits both isoforms.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

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Peer-Reviewed Papers
15

References

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