• USA Home
  • M7431 - Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
M7431 Sigma

Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody produced in mouse

clone 1B3B9, purified immunoglobulin, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Kinase/Phosphatase Biology,
species reactivity   mouse, rat, human
application(s)   immunoprecipitation: 4 μg using denatured ERK-2 from a mouse 3T3 fibroblast lysate
  microarray: suitable
  western blot: 0.5 μg/mL using lysates from human A431 cells, mouse 3T3 fibroblasts or rat L6 cells
clone   1B3B9, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt 42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK1(5594)
mouse ... Mapk1(26413)
rat ... Mapk1(116590)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant mouse MAP kinase.

General description

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.
Anti-MAP Kinase 2 recognizes the 42 kDa MAPK2 encoded by the mapk gene. The antibody may weakly react with MAPK1 (ERK1) and only binds denatured MAP kinase.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide

Application

Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody is suitable for detection by immunoblotting, at a working concentration of 0.5 μg/mL, using whole cell lysates of human A431, mouse 3T3 fibroblasts or rat L6 cells. Detection of MAPK2 (ERK-2) was possible in human colon adenocarcinoma cells, HT-29. Immunoprecipitation applications in mouse 3T3 fibroblasts require a concentration of 4 μg. The antibody may be suitable for protein microarray.

Price and Availability


Detect Your Unseen Protein

All labs need water

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?