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  • M7433 - Anti-phospho-MAP Kinase Kinase 4 (MKK4, SEK, JNKK1) (pThr223) antibody produced in rabbit

M7433 Sigma

Anti-phospho-MAP Kinase Kinase 4 (MKK4, SEK, JNKK1) (pThr223) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphothreonine (pThr),
species reactivity   mouse, human, rat
application(s)   western blot (chemiluminescent): 1:2,000 using sodium chloride and vanadate-treated 293 cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MAP2K4(6416)
mouse ... Map2k4(26398)
biological source   rabbit
conjugate   unconjugated



synthetic phospho-Thr223 peptide corresponding to residues around Thr223 of human SEK1.

General description

SAPK/ERK-1 kinase (SEK1) also called MKK4 or Jun kinase kinase (JNKK) activates the stress activated protein kinase (SAPK). There are other mechanisms by which SAPK may be activated but SEK1 being the immediate upstream activator defines the ensuing protein kinase cascade by the c-Jun transcription factor. Activation of SEK1 occurs through phosphorylation of serine and threonine residues at position 219 and 223 respectively by MEKK. High levels of JNK/SAPK activity are reported to induce apoptosis by the activation of c-Jun. Anti-phospho-MAP Kinase Kinase 4 (MKK4, SEK1, JNKK1 (phosphothreonine 223) detects SEK1/MKK4 protein when activated by phosphorylation at Thr223.

Physical form

Solution in 10 mM HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/mL bovine serum albumin and 50% glycerol.


Does not react with non-phosphorylated MKK4/SEK1 or phosphorylated MKK3, MEK-1 or MEK-2.


Anti-phospho-MAP Kinase Kinase 4 may be used for immunoblotting at a working dilution of 1:2,000 using sodium chloride and vanadate-treated 293 cells.

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