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M7522 Sigma

2-Mercaptoethanol

liquid, BioReagent, suitable for cell culture

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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Properties

vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
product line   BioReagent
form   liquid
expl. lim.   18 %
concentration   14.3 M (pure liquid)
refractive index   n20/D 1.500(lit.)
bp   157 °C(lit.)
solubility   H2O: soluble1 mL/mL
density   1.114 g/mL at 25 °C(lit.)
suitability   suitable for cell culture

Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

This product has been replaced by M3148-SIGMA | 2-Mercaptoethanol for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

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Safety & Documentation

Safety Information

Signal word 
Danger
Target organs 
Heart, Liver
RIDADR 
UN 2966 6.1 / PGII
WGK Germany 
3
RTECS 
KL5600000
Protocols & Articles

Articles

A Biologically-Inspired Electrochemical Half-Cell for Light-Driven Generation of Hydrogen

An economy based on hydrogen has long been touted as one of the most promising routes to a sustainable energy future. Hydrogen gas (H2) is a particularly clean source of energy that can be utilized e...
Rebecca A. Grimme1 and John H. Golbeck*1,2
Material Matters 2008, 3.4, 78.
Keywords: Absorption, Biochemistry, Catalysis, Deposition, Diffusion, Ligands, Mass spectrometry, Metabolism, Methods, Molecular biology, Nucleic acid denaturation, Photosynthesis, Recombination, Reductions, Semiconductor, Support, Type

Peer-Reviewed Papers
15

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