EMAIL THIS PAGE TO A FRIEND

M7522 Sigma

2-Mercaptoethanol

liquid, BioReagent, suitable for cell culture

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

Purchase

Properties

vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
product line   BioReagent
form   liquid
expl. lim.   18 %
concentration   14.3 M (pure liquid)
refractive index   n20/D 1.500(lit.)
bp   157 °C(lit.)
solubility   H2O: soluble1 mL/mL
density   1.114 g/mL at 25 °C(lit.)
suitability   suitable for cell culture

Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

This product has been replaced by M3148-SIGMA | 2-Mercaptoethanol for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Price and Availability


Learn about PharmaGrade

Customers Also Viewed

2-Mercaptoethanol

for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

2-Mercaptoethanol

for electrophoresis

2-Mercaptoethanol

BioUltra, for molecular biology, ≥99.0% (GC)

Safety & Documentation

Safety Information

Signal word 
Danger
Target organs 
Heart, Liver
Hazard Codes (Europe) 
T,N
Risk Statements (Europe) 
Safety Statements (Europe) 
26-36/37/39-45-60-61
RIDADR 
UN 2966 6.1 / PGII
WGK Germany 
3
RTECS 
KL5600000

Protocols & Articles

Articles

A Biologically-Inspired Electrochemical Half-Cell for Light-Driven Generation of Hydrogen

An economy based on hydrogen has long been touted as one of the most promising routes to a sustainable energy future. Hydrogen gas (H2) is a particularly clean source of energy that can be utilized e...
Rebecca A. Grimme1 and John H. Golbeck*1,2
Material Matters 2008, 3.4, 78.
Keywords: Absorption, Biochemistry, Catalysis, Deposition, Diffusion, Ligands, Mass spectrometry, Metabolism, Methods, Molecular biology, Nucleic acid denaturation, Photosynthesis, Recombination, Reductions, Semiconductor, Support, Type

Peer-Reviewed Papers

References

Set your institution to view full text papers.

In several cell types tumour suppressor p53 induces apoptosis largely via Puma but Noxa can contribute. Michalak, EM Cell Death Differ. 15(6), 1019-29, (2008)

Loading...


PD-L1 regulates the development, maintenance, and function of induced regulatory T cells. Francisco LM J. Exp. Med. 206(13), 3015-29, (2009)

Loading...


The generation of live offspring from vitrified oocytes. L. Gabriel Sanchez-Partida et al PLoS ONE 6, e21597, (2011)

Loading...


Integration-free iPS cells engineered using human artificial chromosome vectors. Masaharu Hiratsuka et al PLoS ONE 6, e25961, (2011)

Loading...


Genomewide analysis of PRC1 and PRC2 occupancy identifies two classes of bivalent domains. Ku M, Koche RP, Rheinbay E, et al. PLoS Genet. 4(10), e1000242, (2008)

Loading...


Hematopoietic development from human induced pluripotent stem cells. Lengerke C Ann. N. Y. Acad. Sci. 1176, 219-27, (2009)

Loading...


Phosphoinositide (3,4,5)-triphosphate binding to phosphoinositide-dependent kinase 1 regulates a protein kinase B/Akt signaling threshold that dictates T-cell migration, not proliferation. Caryll Waugh et al Mol. Cell. Biol. 29, 5952-62, (2009)

Loading...


Capillary electrophoresis of seed storage proteins: the separation and identification of microheterogeneous rice glutelin subunits. Katsube-Tanaka T Methods Mol. Biol. 984, 253-61, (2013)

Loading...


A new multistep induction protocol for the transdifferentiation of bone marrow stromal stem cells into GABAergic neuron-like cells. Darabi S, Tiraihi T, Delshad A, et al. Iran. Biomed. J. 17(1), 8-14, (2013)

Loading...


[Investigation of functional groups of Cryptococcus albidus alpha-L-rhamnosidase]. Gudzenko OV and Varbanets' LD Mikrobiol. Z. 74(4), 19-28, (2012)

Loading...


Genetic relationships between Candida albicans strains isolated from dental plaque, trachea, and bronchoalveolar lavage fluid from mechanically ventilated intensive care unit patients. Heo SM, Sung RS, Scannapieco FA, et al. J. Oral Microbiol. 3, doi:10.3402/jom.v3i0.6362, (2011)

Loading...


Stromagenesis During Tumorigenesis: Characterization Of Tumor-associated Fibroblasts And Stroma-derived 3D Matrices. Castelló-Cros, R., and Cukierman, E. Methods Mol. Biol. 522, 275-305, (2009)

Loading...


Enhanced secretion of interferon-gamma by bovine gammadelta T cells induced by coculture with Mycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals. Price SJ Immunology 126(2), 201-8, (2009)

Loading...


Regulation of eumelanin/pheomelanin synthesis and visible pigmentation in melanocytes by ligands of the melanocortin 1 receptor. Le Pape E, Wakamatsu K, Ito S, et al. Pigment Cell Melanoma Res. 21(4), 477-86, (2008)

Loading...


A method for nanofluidic device prototyping using elastomeric collapse. Seung-min Park et al Proc. Natl. Acad. Sci. U. S. A. 106, 15549-54, (2009)

Loading...


Efficacy of liposomal bismuth-ethanedithiol-loaded tobramycin after intratracheal administration in rats with pulmonary Pseudomonas aeruginosa infection. Alhariri M and Omri A Antimicrob. Agents Chemother. 57(1), 569-78, (2013)

Loading...


Mercaptoethanol capped CdSe quantum dots and CdSe/ZnS core/shell: synthesis, characterization and cytotoxicity evaluation. Painuly D, Bhatt A, and Krishnan VK J. Biomed. Nanotechnol. 9(2), 257-66, (2013)

Loading...


On the surface interactions of proteins with lignin. Salas C, Rojas OJ, Lucia LA, et al. ACS Appl. Mater. Interfaces 5(1), 199-206, (2013)

Loading...


A microplate fluorescence assay for DAPA aminotransferase by detection of the vicinal diamine 7,8-diaminopelargonic acid. Mann S, Eveleigh L, Lequin O, et al. Anal. Biochem. 432(2), 90-6, (2013)

Loading...


Scalable culture and cryopreservation of human embryonic stem cells on microcarriers. Ying Nie et al Biotechnol. Prog. 25, 20-31, (2009)

Loading...


The relative efficiency of homology-directed repair has distinct effects on proper anaphase chromosome separation. Laulier C Nucleic Acids Res. 39(14), 5935-44, (2011)

Loading...


Pro-inflammatory cytokine/chemokine production by reovirus treated melanoma cells is PKR/NF-κB mediated and supports innate and adaptive anti-tumour immune priming. Steele, L Mol. Cancer 10, 20, (2011)

Loading...


Cyclin-dependent kinase 5 promotes pancreatic β-cell survival via Fak-Akt signaling pathways. Daval M Diabetes 60(4), 1186-97, (2011)

Loading...


Integrative DNA methylation and gene expression analyses identify DNA packaging and epigenetic regulatory genes associated with low motility sperm. Pacheco SE PLoS ONE 6(6), e20280, (2011)

Loading...


AGEs decrease insulin synthesis in pancreatic β-cell by repressing Pdx-1 protein expression at the post-translational level. Shu T, Zhu Y, Wang H, et al. PLoS ONE 6(4), e18782, (2011)

Loading...


Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice. Jinhong Meng et al PLoS ONE 6, e17454, (2011)

Loading...


Carbon monoxide blocks lipopolysaccharide-induced gene expression by interfering with proximal TLR4 to NF-kappaB signal transduction in human monocytes. Chhikara M PLoS ONE 4(12), e8139, (2009)

Loading...


Endothelial cells in co-culture enhance embryonic stem cell differentiation to pancreatic progenitors and insulin-producing cells through BMP signaling. Talavera-Adame D, Wu G, He Y, et al. Stem Cell Rev. 7(3), 532-43, (2011)

Loading...


Administration of M. leprae Hsp65 interferes with the murine lupus progression. Marengo EB, de Moraes LV, Faria M, et al. PLoS ONE 3(8), e3025, (2008)

Loading...


Impaired fertilizing ability of superoxide dismutase 1-deficient mouse sperm during in vitro fertilization. Tsunoda S, Kawano N, Miyado K, et al. Biol. Reprod. 87(5), 121, (2012)

Loading...


In vitro neuronal induction of adipose-derived stem cells and their fate after transplantation into injured mouse brain. Pavlova G, Lopatina T, Kalinina N, et al. Curr. Med. Chem. 19(30), 5170-7, (2012)

Loading...


Toll-like receptor 2 dependent immunogenicity of glycoconjugate vaccines containing chemically derived zwitterionic polysaccharides. Gallorini S Proc. Natl. Acad. Sci. U. S. A. 106(41), 17481-6, (2009)

Loading...


The influences of reaction conditions on magnetic porous pseudo-carbon paste electrode for schistosoma egg antigen detection. Xu L, Du J, and He N J. Nanosci. Nanotechnol. 13(3), 2302-6, (2013)

Loading...


Rapid formal hydrolysis of peptide-αthioesters. Gates ZP, Stephan JR, Lee DJ, et al. Chem. Commun. (Camb.) 49(8), 786-8, (2013)

Loading...


Structure of a eukaryotic thiaminase I. Kreinbring CA, Remillard SP, Hubbard P, et al. Proc. Natl. Acad. Sci. U. S. A. 111(1), 137-42, (2014)

Loading...


The protein interactions and rheological properties of skim milk heated in the presence of low levels of reducing agent. Nguyen NH, Wong M, Havea P, et al. Food Chem. 138(2-3), 1604-9, (2013)

Loading...


BNP signaling is crucial for embryonic stem cell proliferation. Abdelalim EM PLoS ONE 4(4), e5341, (2009)

Loading...


Reovirus nonstructural protein sigma1s is required for establishment of viremia and systemic dissemination. Boehme KW Proc. Natl. Acad. Sci. U. S. A. 106(47), 19986-91, (2009)

Loading...


Microsomal prostaglandin E synthase type 2 (mPGES2) is a glutathione-dependent heme protein, and dithiothreitol dissociates the bound heme to produce active prostaglandin E2 synthase in vitro. Takusagawa F J. Biol. Chem. 288(14), 10166-75, (2013)

Loading...


Cigarette smoke extract (CSE) delays NOD2 expression and affects NOD2/RIPK2 interactions in intestinal epithelial cells. Aldhous MC, Soo K, Stark LA, et al. PLoS ONE 6(9), e24715, (2011)

Loading...


Reconstitution of Schwannian stroma in neuroblastomas using human bone marrow stromal cells. Wenlin Du et al Am. J. Pathol. 173, 1153-64, (2008)

Loading...


Regulators of cellular levels of histone acetylation in Saccharomyces cerevisiae. Peng W, Togawa C, Zhang K, et al. Genetics 179(1), 277-89, (2008)

Loading...


Multifunctional thiols from the highly selective reaction of mercaptoalcohols with chlorosilanes. Jennings AR and Son DY Chem. Commun. (Camb.) 49(33), 3467-9, (2013)

Loading...


Human augmenter of liver regeneration: probing the catalytic mechanism of a flavin-dependent sulfhydryl oxidase. Schaefer-Ramadan S, Gannon SA, and Thorpe C Biochemistry 52(46), 8323-32, (2013)

Loading...


Differences in Salmonella enterica serovar Typhimurium strain invasiveness are associated with heterogeneity in SPI-1 gene expression. Leann Clark et al Microbiology 157, 2072-83, (2011)

Loading...


Beta-mecaptoethanol suppresses inflammation and induces adipogenic differentiation in 3T3-F442A murine preadipocytes. Guo W, Li Y, Liang W, et al. PLoS ONE 7(7), e40958, (2012)

Loading...


Gene expression analysis of embryonic stem cells expressing VE-cadherin (CD144) during endothelial differentiation. Nikolova-Krstevski V, Bhasin M, Otu HH, et al. BMC Genomics 9, 240, (2008)

Loading...


A point mutation produced a class 3 aldehyde dehydrogenase with increased protective ability against the killing effect of cyclophosphamide. Ho KK Biochem. Pharmacol. 76(5), 690-6, (2008)

Loading...


Carbonic anhydrase inhibitors: the very weak inhibitors dithiothreitol, beta-mercaptoethanol, tris(carboxyethyl)phosphine and threitol interfere with the binding of sulfonamides to isozymes II and IX. Alessio Innocenti et al Bioorg. Med. Chem. Lett. 18, 1898-903, (2008)

Loading...


Oxygen tension modulates differentiation and primary macrophage functions in the human monocytic THP-1 cell line. Grodzki AC, Giulivi C, and Lein PJ PLoS ONE 8(1), e54926, (2013)

Loading...


Generation of diverse neuronal subtypes in cloned populations of stem-like cells. Varga BV BMC Dev. Biol. 8, 89, (2008)

Loading...


Interaction of fish aryl hydrocarbon receptor paralogs (AHR1 and AHR2) with the retinoblastoma protein. Merson RR Aquat. Toxicol. 94(1), 47-55, (2009)

Loading...


Oxidative folding of lysozyme with aromatic dithiols, and aliphatic and aromatic monothiols. Amar S Patel et al Bioorg. Med. Chem. 20, 1020-8, (2012)

Loading...


Transforming growth factor-β-inducible early response gene 1 is a novel substrate for atypical protein kinase Cs. Alemu, E.A., et al. Cell. Mol. Life Sci. 68, 1953-68, (2011)

Loading...


The ATP/DNA ratio is a better indicator of islet cell viability than the ADP/ATP ratio. T.M. Suszynski et al Transplant. Proc. 40, 346-50, (2009)

Loading...


Differentiation of human adipose-derived mesenchymal stem cell into insulin-producing cells: an in vitro study. Moshtagh PR, Emami SH, and Sharifi AM J. Physiol. Biochem. 69(3), 451-8, (2013)

Loading...


Pathogenic MOG-reactive CD8+ T cells require MOG-reactive CD4+ T cells for sustained CNS inflammation during chronic EAE. Bettini M J. Neuroimmunol. 213(1-2), 60-8, (2009)

Loading...


A protocol for stripping and reprobing of Western blots originally developed with colorimetric substrate TMB. Kar P, Agnihotri SK, Sharma A, et al. Electrophoresis 33(19-20), 3062-5, (2012)

Loading...


Improved performance of nanowire-quantum-dot-polymer solar cells by chemical treatment of the quantum dot with ligand and solvent materials. Nadarajah A, Smith T, and Könenkamp R Nanotechnology 23(48), 485403, (2012)

Loading...


Expression of RHOGTPase regulators in human myometrium. O'Brien M Reprod. Biol. Endocrinol. 6, 1, (2008)

Loading...


Caveolin-1 regulates neural differentiation of rat bone mesenchymal stem cells into neurons by modulating Notch signaling. Wang S, Kan Q, Sun Y, et al. Int. J. Dev. Neurosci. 31(1), 30-5, (2013)

Loading...


Use of long-term cultured embryoid bodies may enhance cardiomyocyte differentiation by BMP2. Kim YY Yonsei Med. J. 49(5), 819-27, (2008)

Loading...


Insulin related compounds and identification. Min CK, Lee JW, Kim CK, et al. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 908, 105-12, (2012)

Loading...


A β-mercaptoethanol-modified enzyme-linked immunosorbent assay for diagnosis of canine visceral leishmaniasis. Barral-Veloso L, Semião-Santos SJ, de Andrade PP, et al. J. Vet. Diagn. Invest. 25(2), 239-42, (2013)

Loading...


Racemization of the aspartic acid residue of amyloid-β peptide by a radical reaction. Tambo K, Yamaguchi T, Kobayashi K, et al. Biosci. Biotechnol. Biochem. 77(2), 416-8, (2013)

Loading...


Micro-emulsion synthesis, surface modification, and photophysical properties of Zn(1-x) Mn(x)S nanocrystals for biomolecular recognition. Mohagheghpour E, Moztarzadeh F, Rabiee M, et al. IEEE Trans. Nanobioscience 11(4), 317-23, (2012)

Loading...


The effects of bestatin on humoral response to sheep erythrocytes in non-treated and cyclophosphamide-immunocompromised mice. Lis M, Szczypka M, Suszko A, et al. Immunopharmacol. Immunotoxicol. 35(1), 133-8, (2013)

Loading...


Inhibition of cell division in mouse B-cell hybridomas: an overlooked property of 2-mercaptoethanol and its impact on in vitro antibody production. Abebe M, Dirieh Y, et al. Hybridoma 31, 240-245, (2012)

Loading...


Alteration of GI symptoms in a cow with Johne disease by the dietary organosulfur, 2-mercaptoethanol. Click RE Virulence 3(6), 543-5, (2012)

Loading...


Differentiation of neural precursors and dopaminergic neurons from human embryonic stem cells. Zhang XQ and Zhang SC Methods Mol. Biol. 584, 355-66, (2010)

Loading...


Merck 14,5869

Beil. 1,IV,2428

Aldrich MSDS 1, 1162:C / Corp MSDS 1 (2), 2209:A / FT-IR 2 (1), 415:B / FT-IR 1 (1), 262:B / FT-NMR 1 (1), 422:B / IR-Spectra (2), 138:E / IR-Spectra (3), 154:F / NMR-Reference 2 (1), 228:B / RegBook 1 (1), 281:L / Sax 6, 1744 / Sigma FT-IR 1 (2), 700:C / Structure Index 1, 38:D:7 / Vapor Phase 3, 344:C

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?