M7522 Sigma

2-Mercaptoethanol

liquid, BioReagent, suitable for cell culture

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

Properties

vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
product line   BioReagent
form   liquid
expl. lim.   18 %
concentration   14.3 M (pure liquid)
refractive index   n20/D 1.500(lit.)
bp   157 °C(lit.)
solubility   H2O: soluble1 mL/mL
density   1.114 g/mL at 25 °C(lit.)
suitability   suitable for cell culture

Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Price and Availability

Customers Also Viewed

2-Mercaptoethanol

≥99.0%

2-Mercaptoethanol

for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

2-Mercaptoethanol

for electrophoresis

2-Mercaptoethanol

BioUltra, for molecular biology, ≥99.0% (GC)

Dimethyl sulfoxide

Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?