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M7528 Sigma

Medium 199

HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Properties

Related Categories Cell Culture, Classic Media and Salts, Medium 199 More...
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
pH range   >7.2
suitability   suitable for cell culture
components   HEPES: 25 mM
L-glutamine: no
NaHCO3: yes
sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
storage temp.   2-8°C

Description

Application

Originally developed as a completely defined media formulation for the culture of primary explants. These media have broad applicability, particularly for non-transformed cells and are widely used for vaccine production, primary pancreatic explants, and lens tissues.

Reconstitution

Supplement with 0.1 g/L L-glutamine.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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