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  • M7556 - Anti-MAP Kinase 2 (ERK-2) antibody produced in rabbit

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M7556 Sigma

Anti-MAP Kinase 2 (ERK-2) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution, ~1 mg/mL

Synonym: Anti-ERK-2

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   human, mouse, rat
application(s)   immunoprecipitation: 5 μg using 0.5-1 mg of a mouse 3T3 fibroblast lysate
  western blot: 0.5-2 μg/mL using human A431 cells, mouse 3T3 fibroblasts or rat L6 cells
clone   polyclonal
concentration   ~1 mg/mL
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK1(5594)
mouse ... Ephb2(13844), Mapk1(26413)
rat ... Mapk1(116590)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

recombinant mouse MAP Kinase 2 (MAPK2, ERK-2).

General description

Mitogen-activated protein kinases (MAPKs) regulate a wide range of functions by modulating cellular signal transduction. These functions include cellular growth, differentiation, movement and apoptosis. Conventional MAPKs comprise of the ERK1/2, p38, JNK and ERK5 kinase families. ERK1 and ERK2 are highly conserved serine/threonine kinases that are responsive to growth factors
Anti-MAP Kinase 2 (ERK-2) antibody is specific for MAPK2 (42 kDa) in mice, humans and rats. This antibody does not cross react with MAP kinase 1 (ERK-1) nor does it interfere with the kinase activity.

Physical form

Solution in 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and ≤ 0.1% sodium azide

Application

Anti-MAP Kinase 2 (ERK-2) antibody is suitable for use in western blot (0.5-2 μg/mL using human A431 cells, mouse 3T3 fibroblasts or rat L6 cells). The product can also be used for immunoprecipitation (5 μg, using 0.5-1 mg of a mouse 3T3 fibroblast lysate).

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