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M7653 Sigma

Medium 199

With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Properties

Related Categories Cell Culture, Classic Media and Salts, Medium 199 More...
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
components   HEPES: no
phenol red: yes
NaHCO3: yes
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
storage temp.   2-8°C

Description

Application

Originally developed as a completely defined media formulation for the culture of primary explants. These media have broad applicability, particularly for non-transformed cells and are widely used for vaccine production, primary pancreatic explants, and lens tissues.

General description

Modification of the original Medium 199.Earle's salts are replaced with Hanks' salts. Hanks' salts use a lower concentration of bicarbonate and different concentrations and salt forms of the essential inorganic ions.

Reconstitution

Supplement with 0.1 g/L L-glutamine.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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