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M7683 Sigma

Anti-phospho-MAP Kinase Kinase 1/2 (pSer217/221) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-phospho-MEK 1/2 (pSer217/221)

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Alzheimer′s Disease, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
species reactivity   mouse, human, rat
application(s)   flow cytometry: 1:50 using PMA treated human peripheral blood lymphocytes and Jurkat cells.
  immunocytochemistry: 1:1,000 using HeLa cells
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections from human breast carcinoma
  immunoprecipitation: 1:50
  western blot: 1:1,000 using serum-treated NIH-3T3 cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
mol wt   antigen mol wt 45 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MAPK1(5594)
mouse ... Mapk1(26413)
rat ... Mapk1(116590)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic phosphopeptide Ser217/221 corresponding to residues around Ser217/221 of human MEK 1/2.

General description

MAP Kinase Kinase (MAPKK, MEK1 and MEK2) belong to a family of enzymes that activates their substrate by dual phosphorylation at threonine and tyrosine residues. MEK1 and MEK2 have 86% homology in their catalytic domain. Activation of MEK1/2 is mediated in mitogen-stimulated cells by Raf-1 kinase. MEK1/2 then activates ERK1/2 leading to the activation of several downstream targets that contribute to cell proliferation, apoptosis and motility. In addition to ERK1/2, there may be non-catalytic effectors of MEK1/2. MEK isoforms appear to be widely expressed in the central nervous system, thymus, spleen, heart, lung, and kidney. Active forms of MEK1/2 are sufficient for the transformation of NIH3T3 cells of the differentiation of PC-12 cells.
The antibody specifically recognizes MEK1/2 (45 kDa) only when phosphorylated at Ser217 and Ser221. It reacts strongly with MEK1/2 phosphorylated only at Ser217, but less when phosphorylated only at Ser221. It shows not cross reaction with any other related family members including SEK.

Physical form

Solution in 10 mM HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol.

Specificity

Detects MEK 1/2 when phosphorylated at Ser217 and Ser221. It does not react with non-phosphorylated MEK 1/2, SEK (MKK4), MKK3, or MKK6.

Application

Anti-phospho-MAP Kinase Kinase 1/2 (MEK1/2) has a wide range of applications. The recommended working dilution for immunoblotting in serum treated NIH3T3 cells is 1:1000. For immunoprecipitation, a working dilution of 1:50 may be used. For detection by immunohistochemistry in paraffin-embedded human breast carcinoma sections, antibody dilution of 1:100 may be used. For immunocytochemistry (immunofluorescence), in HeLa cells, a working dilution of 1:1000 may be used.

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