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M7786 Sigma

Monoclonal Anti-Myosin (Smooth) antibody produced in mouse

clone hSM-V, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology,
species reactivity   canine, rabbit, pig, guinea pig, human, chicken
application(s)   immunohistochemistry (frozen sections): suitable using human and animal tissue
  immunohistochemistry: 1:500 using methacarn-fixed, paraffin-embedded sections of human and animal tissue
  immunoprecipitation: suitable
  western blot: suitable
clone   hSM-V, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 200-204 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MYH11(4629)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human uterus smooth muscle extract.

General description

Contractile proteins, which are involved with motility functions, have been reported to be ubiquitous in eukaryotic cells. Two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. Myosin (~500 kDa) containing two identical heavy chains (~200 kDa each) and four light chains (15-26 kDa). Myosin molecules consist of two major regions: tail (rod) and heads. They aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Myosin molecules spontaneously assemble into filaments in solutions of physiologic ionic strength and pH. In fact, the thick filament consists mainly of myosin molecules. Myosin is activated by the enzyme ATPase. This activation is the immediate source of the free energy that drives muscle contraction. It binds to the polymerized form of actin, the major constituent of the thin filament. Myosin is generally assumed to be a very specific and reliable smooth muscle marker, provided that the antibody used is specific for smooth muscle myosin and does not cross-react with skeletal, cardiac or non-muscle myosins.

Mouse monoclonal clone hSM-V anti-Myosin (Smooth) antibody reacts in immunoblotting assays with myosin heavy chain polypeptides of 204 and 200 kDa (SM-1 and SM-2). It does not cross-react with skeletal, cardiac or non-muscle myosin. The antibody stains vascular and visceral smooth muscle cells, as well as cells that have smooth muscle-like characteristics (myofibroblasts and myoepithelial cells). It does not recognize epithelial, endothelial or connective tissue fibroblast cells. The product reacts with smooth muscle tissue from human, dog, rabbit, pig, guinea pig, and chicken.

Application

Mouse monoclonal clone hSM-V anti-Myosin (Smooth) antibody can be used to identify smooth muscle cells as well as cells that express certain smooth muscle-like features, i.e. myofibroblasts and myoepithelial cells. It is useful in the identification and localization of myosin in smooth muscle elements, in the diagnosis and classification of mesenchymal tumors and in studies on myosinexpression in normal and malignant cell proliferations. The antibody may be used in immunoblotting, immunoprecipitation, and immunohistochemical staining of methacarn-fixed, paraffin-embedded, or frozen tissue sections.

Price and Availability


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