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M7905 Sigma

Monoclonal Anti-Myosin Light Chain Kinase antibody produced in mouse

clone K36, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, MUE-MZ,
species reactivity   avian, mammals
application(s)   immunoprecipitation: suitable
  microarray: suitable
  western blot: 1:10,000
clone   K36, monoclonal
antibody form   ascites fluid
isotype   IgG2b
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MYLK(4638)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

chicken gizzard myosin light chain kinase.

General description

Myosin Light Chain Kinase (MLCK) is a calcium/calmodulin dependent enzyme (approx.160 kD). It has a pivotal role in phosphorylating myosin regulatory light chains and hence facilitates its interaction with actin filaments for smooth muscle contraction. Monoclonal anti-myosin light chain kinase antibody can be used for studying the function of the enzyme and its interactions with other cell components. It can also be used in microarray and immunoprecipitation. Mouse anti-myosin light chain kinase antibody reacts specifically with myosin light chain kinase present in smooth muscle of chicken gizzard, turkey gizzard, and pig stomach. This product also shows reactivity for myosin light chain kinase of non-muscle cells like cultured fibroblasts.

Application

Monoclonal anti-myosin light chain kinase antibody can be used as a primary antibody in immunohistochemistry. It can also be used in immunoblot analysis and flow cytometry. Further, the product can also be used in western blotting (diluted 1:10,000).

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