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  • M7927 - Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit

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M7927 Sigma

Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   human, mouse, rat
application(s)   immunoprecipitation: 5 μg using NIH 3T3 mouse fibroblast lysates
  microarray: suitable
  western blot: 1:5,000 using NIH 3T3 mouse fibroblast lysate or rat brain extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen, ERK-1 mol wt 44 kDa
  antigen, ERK-2 mol wt 42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK3(5595)
mouse ... Mapk3(26417)
rat ... Mapk3(50689)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 351-368 of human ERK-1.

General description

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK1 (p44) and ERK2 (p42) require the dual phosphorylation in the catalytic kinase domain by MEKs for their full activity. ERK1 is phosphorylated on Tyr204 and Thr202, and ERK2 on Tyr187 and Thr185. ERK1 and 2 may also undergo autophosphorylation on these residues. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.
Anti-MAP Kinase (ERK-1, 351-368) reacts specifically with ERK-1 and ERK-2 (44 and 42 kDa) derived from rat brain and mouse fibroblast cell extract.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide

Specificity

By immunoblotting, the antibody reacts with ERK-1 and ERK-2.

Application

A minimum working dilution of 1: 5,000 is determined by immunoblotting using a rat brain cytosolic extract or a lysate of cultured NIH 3T3 mouse fibroblasts. The antibody may be used in immunoprecipitation at a concentration of 5 μg with protein A-agarose and 25 μg of a lysate of cultured NIH 3T3 mouse fibroblasts
The antibody may be used for detection of ERK-1 and ERK-2 in human embryonic kidney epithelial 293T, murine long bone-derived osteocytic cell line MLO-Y4, porcine brain extract and Chinese hamster lung fibroblast cell line CCL39

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