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  • M7939 - Anti-Myosin IIB, non-muscle antibody produced in rabbit

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M7939 Sigma

Anti-Myosin IIB, non-muscle antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   rat, human, canine
application(s)   western blot (chemiluminescent): 1:200 using whole cell extracts of cultured dog MDCK kidney cells, cultured rat NRK cells, and cultured human Jurkat cells.
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~200 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Myh10(79433)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

Synthetic peptide corresponding to amino acid residues 1965-1976 of the heavy chain of human myosin IIB (nonmuscle) conjugated to KLH. This sequence is identical in rabbit and differs by one and two amino acids from the respective bovine and rat sequences.

General description

Myosins are actin-dependent motors that have 15 classes based on their phylogenetics . Myosin IIb regulates memory and synaptic plasticity functions. Nonmuscle myosin IIb has been implicated in fibroblast movement and erythroblast enucleation . Anti-Myosin IIB, non-muscle antibody is specific for the heavy chain of human myosin IIB (nonmuscle). The antibody also reacts with myosin in dogs and rats. Staining of myosin IIB (nonmuscle) by immunoblotting is inhibited by the immunizing peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Myosin IIB, non-muscle antibody is suitable for use in chemiluminescent western blot (1:200, using whole cell extracts of cultured dog MDCK kidney cells, cultured rat NRK cells, and cultured human Jurkat cells). It may also be used for immunoblot (approx. 200kDa, additional bands may be detected in some extract preparations).

Price and Availability


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