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M8159 Sigma

Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) antibody produced in mouse

clone MAPK-YT, ascites fluid

Synonym: Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2)

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Alzheimer′s Disease, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   yeast, Xenopus, human, rat, bovine, Caenorhabditis elegans, Drosophila, mouse, hamster
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 1:10,000 using rat brain extract
clone   MAPK-YT, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen ERK-1 mol wt 44 kDa
  antigen ERK-2 mol wt 42 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK1(5594), MAPK3(5595)
mouse ... Mapk1(26413), Mapk3(26417)
rat ... Mapk1(116590), Mapk3(50689)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide HTGFLpTEpYVAT corresponding to the phosphorylated form of the ERK-activation loop.

General description

MAP kinase (MAPK, mitogen-activated protein kinase, also termed ERK, extracellular regulated protein kinase), consists of a family of protein kinases which are considered to play a crucial role in various signal transduction pathways leading signals of growth factor, as well as G protein-coupled receptors to their intracellular targets. MAP kinase was shown to regulate several cellular processes among them proliferation, differentiation, cellular morphology and oncogenesis.
Molecular cloning has established that MAP kinase (ERKs) consists of at least four isoforms: ERK-1 (p44mapk), ERK-2 (p42mapk), ERK-3, and ERK-5.
Activation of ERK-1 and ERK-2 in mitogen-stimulated cells is directly mediated by MAP kinase kinase (MAPKK or MEK), a dual-specificity protein kinase, which phosphorylates both threonine and tyrosine residues in the regulatory sites of MAP kinase. Following activation, MAP kinase phosphorylates several nuclear targets, including transcription factors as well as membrane and cytoskeletal proteins. Termination of MAP kinase signalling appears to be mediated by MAP kinase phosphatase, MKP-1, a dual specificity Thr/Tyr phosphatase which dephosphorylates and inactivates MAP kinase. MAP kinase isoforms appear to be widely expressed, in the central nervous system, thymus, spleen, heart, lung, kidney, and are expressed in high levels in PC12 cells and in fibroblasts.

Specificity

The antibody reacts specifically with the diphosphorylated form of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated or the monophosphorylated forms of MAP kinase or the diphosphorylated forms of JNK and p38 MAP kinase. The epitope recognized by the antibody contains the phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase.

Application

Antibodies that react specifically with the active form of MAP kinase are useful for the study of the specific activation requirements, differential tissue expression, and intracellular localization of the active form of MAP kinase in normal and neoplastic tissue.
Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) may be used for the localization of the active, dually-phosphorylated, form of MAP kinase using various immunochemical assays such as immunoblotting of cultured cells and tissue extracts, ELISA, immunocytochemistry, immunoprecipitation, and in immunohistochemistry (formalin and formaldehyde-fixed sections). Reactivity has been observed with human, bovine, rat, mouse, Drosophila, Spodoptera frugiperda, and yeast.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

Alzheimer's Disease

Alzheimer's disease (AD) is the most common cause of dementia in the elderly and is characterized by gradual loss of cognitive functions. Hallmark pathohistological findings of AD include widespread ...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 4–8
Keywords: Alzheimer Disease, Gene expression, Genetic, Genetics, Inflammation, Metabolism, Phosphorylations

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Peer-Reviewed Papers
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