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M8177 Sigma

Monoclonal Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) antibody produced in mouse

~2 mg/mL, clone P38-TY, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-MAP Kinase, p38, Activated

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies for Parkinson’s Disease Research,
species reactivity   mouse, rat, human
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1 μg/mL using whole cell extract from a rat fibroblast cell line, Rat1, activated with sorbitol
clone   P38-TY, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK14(1432)
mouse ... Mapk14(26416)
rat ... Mapk14(81649)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

Synthetic peptide HTDDEMpTGpYVATR corresponding to the phosphorylated form of p38 MAP kinase activation loop, coupled to KLH.

General description

P38 is a mitogen-activated protein kinase (MAPK) that belongs to serine/threonine protein kinases family and is a member of the stress-activated protein kinases (SAPK). P38 signaling pathway functions in control of inflammatory responses, apoptosis, cell proliferation and differentiation. Dually phosphorylated p38 (i.e (Thr180) and (Tyr182)) is a 43kD endogenous protein that belongs to High Osmolarity Glycerol response kinase (HOG). Monoclonal anti-p38 MAP kinase activated (diphosphorylated p38) antibody can be used in western blotting (diluted 1:500 in 0.1% Tween 20 per 0.1 mol/L Tris buffered saline) . It can also be used in immunocytochemistry. Mouse anti-p38 MAP kinase activated (diphosphorylated p38) antibody reacts specifically with an epitope that contain the phosphorylated threonine and tyrosine residues within the regulatory site of active p38 MAP kinase (Thr180-Gly181-Tyr182). The antibody also reacts with the active doubly-phosphorylated form of p38 MAPK and its related isoforms but not with the non-phosphorylated, monophosphorylated and diphosphorylated forms of JNK and ERK-MAPK or the non-phosphorylated and mono-phosphorylated forms of the p38 MAPK molecule. Further, the product has shown reactivity for rat, mouse and human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Reacts specifically with the active diphosphorylated form of p38 MAP kinase and its closely related isoforms. Does not recognize the non-phosphorylated and mono­phosphory­lated forms of the p38 MAP kinase molecule, or the non-phosphorylated, monophosphorylated and diphosphorylated forms of JNK and ERK-MAP kinases. The epitope recognized by the antibody contains the phosphorylated threonine and tyrosine residues within the regulatory site of active p38 MAP kinase (Thr180-Gly181-Tyr182).

Preparation Note

From a culture supernatant of bioreactor grown hybridoma.

Application

Monoclonal anti-p38 MAP kinase activated (diphosphorylated p38) antibody can be used as a primary antibody in Immunohistochemistry (diluted 1:200) . It can also be used in microarray and indirect ELISA.

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